Application
Western blot
Sample
Human Cell lysate - whole cell (hES and cancer cell lines)
Gel Running Conditions
Reduced Denaturing (10% gel)
Loading amount
100000 cells
Specification
hES and cancer cell lines
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 16°C
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk
Dilution
1/1000
Detection
Detection method
ECL+
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 100 kDa Non-specific: 35 kDa
Positive control
HUES7, MCF7 and Met5a human cell lines.
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投稿 Aug 18 2015