Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HeLa)
Specification
HeLa
Fixative
Methanol
Permeabilization
Yes - 0.5% Triton-X100 in PBS
Other product details
Dilution
1/200
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: PBS
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
Asynchronous HeLa cells were either paraformaldehyde (4%, 10min) or methanol (-20'C, 10min) fixed, immunofluorescently labeled and counterstained with DAPI. The accompanying image is a low resolution image of methanol fixed HeLa cells immunofluorescently labeled with anti-MSH2 and show a clear nuclear localization.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Kirk Mcmanus
Verified customer
投稿 Nov 30 2007