Application
Western blot
Sample
Human Cell lysate - other (lung carcinoma)
Loading amount
25 µg
Specification
lung carcinoma
Gel Running Conditions
Reduced Denaturing (7.5%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/250
Incubation time
2 hour(s) and 30 minute(s) · Temperature: 25°C · Diluent: TBS+tween
Secondary antibody
Name
Dilution
1/25000
Detection
Detection method
ECL+
Exposure
5 minute(s) and 0 second(s)
Bands
Specific: 200 kDa
Additional data
Abcam response
There are several isoforms according to SwissProt ("4 named isoforms produced by alternative splicing. Additional isoforms seem to exist"), one of which may account for the band observed at 50 kD.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Aug 19 2009