MRP2 antibody [M2 III-6] (ab3373)

Product Name 

MRP2 antibody [M2 III-6]

Product type 

Primary antibodies

Description 

Mouse monoclonal [M2 III-6] to MRP2

Immunogen 

Bacterial fusion protein of human MRP 2 containing the carboxy-terminal region (202-)

Reacts with 
(species key)

Hu, Rat

Specificity 

This antibody detects MRP 2. It does not cross-react with human MDR 1, MRP 1, MRP 3 or MRP 5 gene products.

Epitope 

This antibody reacts with an internal epitope of MRP 2.

Tested applications 
(see key)

Flow Cyt, ICC, IHC-Fr, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
Flow Cyt: Use at an assay dependent dilution (PMID 18929567).
ICC: Use at an assay dependent dilution.
IP: Use at an assay dependent dilution (from PubMed:16687572).
IHC-P: 1/200. See Abreview. Abreview indicates that several methods of antigen retreival are suitable for this antibody, but heat mediated Ag retrieval in citrate buffer gave optimal results.

IHC-Fr: Use at an assay dependent dilution.
WB: 1/20 - 1/50 (Scheffer GL et al. 2000).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Cellular localization 

Membrane; multi pass membrane protein.

Research areas 

Cancer >> Drug resistance >> MRP-related proteins
Signal Transduction >> Metabolism >> Plasma Membrane >> Channels

Relevance 

MRP2 mediates hepatobiliary excretion of numerous organic anions and may function as a cellular cisplatin transporter. The highest expression is found in liver. MRP2 is a member of the MRP family of multidrug resistance related proteins, and MRP2 overexpression has been observed in a subset of cisplatin resistant cell lines. Defects in MRP2 are the cause of Dubin-Johnson syndrome, an autosomal recessive disorder characterized by conjugated hyperbilirubinemia, an increase in the urinary excretion of coproporphyrin isomer I, deposition of melanin like pigment in hepatocytes, and prolonged retention of sulfobromophthalein, but otherwise normal liver function.

Database links 

Raised in 

Mouse

Clonality 

Monoclonal

Clone number 

M2 III-6

Isotype 

IgG2a

Storage buffer 

PBS with 1% BSA and 0.1% sodium azide

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.250 mg/ml

Storage instructions 

Store at +4°C.

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MRP2 antibody [M2 III-6] images:

References for MRP2 antibody [M2 III-6] (ab3373)

  This product has been used in: (two most recent references)

Mannermaa E et al. Efflux protein expression in human retinal pigment epithelium cell lines. Pharm Res 26:1785-91 (2009). WB; Human. PubMed: 19384462

Fan J et al. Up-regulation of transporters and enzymes by the vitamin D receptor ligands, 1alpha,25-dihydroxyvitamin D3 and vitamin D analogs, in the Caco-2 cell monolayer. J Pharmacol Exp Ther 330:389-402 (2009). PubMed: 19414624

See all 5 publication references for this product.


If you publish research using ab3373 please let us know so that we can cite the reference on this datasheet.

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