Application
Western blot
Sample
Mouse Tissue lysate - whole (Hippocampal Tissues)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
100 µg
Specification
Hippocampal Tissues
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
8 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBST
Dilution
1/1000
Secondary antibody
Dilution
1/2000
Name
Non-Abcam antibody was used: HRP-conjugated secondary antibody
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
2 hour(s), 0 minute(s) and 0 second(s)
Bands
Specific: 91 kDa
Additional data
Additional Notes
PVDF membrane was used for blotting, washings of blots were performed by PBST and blot was developed using Immobilon Western Chemiluminescent HRP Substrate of Millipore.
PubMed ID
27431094: View
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
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投稿 Mar 31 2017