Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (Neutrophils and Monocytes)
Specification
Neutrophils and Monocytes
Permeabilization
Yes - 2%BSA + 0.2% tritonX100 in PBS
Fixative
Paraformaldehyde
Other product details
Incubation time
25 minute(s) · Temperature: 23°C · Diluent: 2%BSA + 0.2% tritonX100 in PBS
Dilution
1/200
Secondary antibody
Name
Non-Abcam antibody was used: Invitrogen Donkey anti-rabbit IgG A10042
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 568
Host species: Donkey
Clonality: Polyclonal
Conjugation: Alexa Fluor 568
Dilution
1/1000
Additional data
Additional Notes
Red : MMP9/Gelatinase
Blue : DAPI
Thioglycollate elicited 12 hr PEC (neutrophils and monocytes) from mice on a B6 background.
Cells were spun down onto slides, fixed for 20 minutes (4%PFA), washed once, stained 25 minutes (2%BSA +0.2% tritonX100) with primary 1/200, washed once, stained with secondary 25 minutes 1/1000, washed once, mounted with DAPI Prolong and imaged the following day.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 May 13 2014