Application
Western blot
Sample
Human Cell lysate - whole cell (NUGC3 gastric cancer cell)
Gel Running Conditions
Reduced Denaturing (10%)
Loading amount
10 µg
Treatment
100, 200 nM PMA for 24 hrs
Specification
NUGC3 gastric cancer cell
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA
Dilution
1/1000
Secondary antibody
Dilution
1/5000
Name
Non-Abcam antibody was used: santa cruz
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Negative control
non-treated NUGC3 cells
Exposure
15 second(s)
Bands
Specific: 90 kDa Non-specific: NO kDa
Positive control
PMA treated NUGC3 cells for 24 hrs
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Jooyoung Im
Verified customer
投稿 Jun 18 2018