Application
Western blot
Sample
Rat Cell lysate - whole cell (rat vascular smooth muscle cells)
Loading amount
100000 cells
Specification
rat vascular smooth muscle cells
Gel Running Conditions
Reduced Denaturing
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C
Other product details
Dilution
1/1000
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 24°C · Diluent: 5% BSA in 0.1% Tween20/PBS
Secondary antibody
Name
Non-Abcam antibody was used: anti rabbit
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Donkey
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/2000
Detection
Detection method
ECL primer
Exposure
30 second(s)
Bands
Specific: 37, 15 kDa
Additional data
Additional Notes
full length protein around 55 kd, upon activation degraded as 37 and 15 kd
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投稿 Feb 05 2013