Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rhesus monkey Tissue sections (Colon)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: High pH followed by low pH buffer
Permeabilization
Yes - 0.1% Triton-X-100
Specification
Colon
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C
Fixative
Z-fix
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 10 mM Tris and 150 mM NaCl
Dilution
1/50
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Goat Anti-Rabbit Conjugated (H+L) to Alexa-488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Additional data
Additional Notes
We used heat mediated antigen retrieval using high and low pH buffers and also tried incubating the primary antibody (1 in 50 dilution) for 2 hr and still did not see any signal.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Sep 30 2015