Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Pig Tissue sections (abdominal aorta)
Antigen retrieval step
Enzymatic - Buffer/Enzyme Used: Proteinase k
Permeabilization
No
Specification
abdominal aorta
Blocking step
SuperBlock™ (PBS) Blocking Buffer (37515) as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 25°C
Fixative
Paraformaldehyde
Other product details
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Dako Antibody Diluent (s302283)
Dilution
1/200
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Millipore DC03L | Goat Anti-Rabbit IgG Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Additional data
Additional Notes
after Secondary antibody
- ABC reagent (vector PK-6100), 30min, 25 °C
- AEC Substrate (Genemed, 10-0005)
- Counterstain with hematoxylin, 10sec
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Sheu Yu Shine
Verified customer
投稿 Jul 14 2015