MMP12 Inhibitor Screening Assay Kit (Colorimetric) (ab139441)
Key features and details
- Assay type: Enzyme activity
- Detection method: Colorimetric
- Platform: Microplate reader
- Sample type: Inhibitor compounds
製品の概要
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製品名
MMP12 Inhibitor Screening Assay Kit (Colorimetric)
MMP12 キット 製品一覧 -
検出方法
Colorimetric -
サンプルの種類
Inhibitor compounds -
アッセイタイプ
Enzyme activity -
製品の概要
Abcam's MMP12 Inhibitor Screening Assay Kit (Colorimetric) (ab139441) is a complete assay system designed to screen MMP12 inhibitors using a thiopeptide as a chromogenic substrate (Ac-PLG-[2-mercapto-4-methyl-pentanoyl]-LG-OC2H5). The MMP cleavage site peptide bond is replaced by a thioester bond in the thiopeptide. Hydrolysis of this bond by an MMP produces a sulfhydryl group, which reacts with DTNB [5,5’-dithiobis(2-nitrobenzoic acid), Ellman’s reagent] to form 2-nitro-5-thiobenzoic acid, which can be detected by its absorbance at 412 nm (e=13,600 M-1cm-1 at pH 6.0 and above). The assays are performed in a convenient 96-well microplate format.
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特記事項
This kit is useful to screen inhibitors of MMP12, a potential therapeutic target. The MMP inhibitor NNGH is also included as a prototypic control inhibitor.
Thiol inhibitors should not be used with this kit, as they may interfere with the colorimetric assay.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Please refer to protocols. -
内容 1 x 96 tests 96-well Clear Microplate (1/2 Volume) 1 unit Colorimetric Assay Buffer 1 x 20ml MMP Inhibitor 1 x 50µl MMP Substrate 1 x 50µl MMP12 Enzyme (Human, Recombinant) 1 x 14µl -
研究分野
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機能
May be involved in tissue injury and remodeling. Has significant elastolytic activity. Can accept large and small amino acids at the P1' site, but has a preference for leucine. Aromatic or hydrophobic residues are preferred at the P1 site, with small hydrophobic residues (preferably alanine) occupying P3. -
組織特異性
Found in alveolar macrophages but not in peripheral blood monocytes. -
配列類似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
ドメイン
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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別名
- EC 3.4.24.65
- HME
- Macrophage elastase
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データシートおよび資料
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SDS download
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Datasheet download
参考文献 (0)
ab139441 は論文での使用が確認できていません。