Anti-MMP11 抗体 (ab53143)
Key features and details
- Rabbit polyclonal to MMP11
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MMP11 antibody
MMP11 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MMP11 -
由来種
Rabbit -
アプリケーション
適用あり: IHC-P, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthesized peptide derived from human MMP11.
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ポジティブ・コントロール
- WB: A549 cell extract
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C. -
バッファー
pH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol (glycerin, glycerine), 49.48% PBS, 0.5% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
The antibody was affinity purified from rabbit antiserum by affinity chromatography using epitope specific immunogen. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53143の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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IHC-P | (1) |
Use a concentration of 2 µg/ml.
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WB |
1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 55 kDa).
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特記事項 |
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IHC-P
Use a concentration of 2 µg/ml. |
WB
1/500 - 1/1000. Detects a band of approximately 62 kDa (predicted molecular weight: 55 kDa). |
ターゲット情報
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機能
May play an important role in the progression of epithelial malignancies. -
組織特異性
Specifically expressed in stromal cells of breast carcinomas. -
配列類似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
ドメイン
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
翻訳後修飾
The precursor is cleaved by a furin endopeptidase. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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参照データベース
- Entrez Gene: 4320 Human
- Entrez Gene: 17385 Mouse
- Entrez Gene: 25481 Rat
- Omim: 185261 Human
- SwissProt: P24347 Human
- SwissProt: Q02853 Mouse
- SwissProt: Q499S5 Rat
- Unigene: 143751 Human
see all -
別名
- Matrix Metalloproteinase 11 antibody
- Matrix metalloproteinase-11 antibody
- MMP-11 antibody
see all
画像
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All lanes : Anti-MMP11 antibody (ab53143) at 1/500 dilution
Lane 1 : A549 cell extract with no peptide
Lane 2 : A549 cell extract with peptide
Predicted band size: 55 kDa
Observed band size: 62 kDa why is the actual band size different from the predicted? -
Ab53143 staining Human normal placenta. Staining is localised to the extracellular matrix (secreted).
Left panel: with primary antibody at 2 ug/ml. Right panel: isotype control.
Sections were stained using an automated system DAKO Autostainer Plus , at room temperature. Sections were rehydrated and antigen retrieved with the Dako 3-in-1 AR buffers EDTA pH 9.0 in a DAKO PT Link. Slides were peroxidase blocked in 3% H2O2 in methanol for 10 minutes. They were then blocked with Dako Protein block for 10 minutes (containing casein 0.25% in PBS) then incubated with primary antibody for 20 minutes and detected with Dako Envision Flex amplification kit for 30 minutes. Colorimetric detection was completed with diaminobenzidine for 5 minutes. Slides were counterstained with Haematoxylin and coverslipped under DePeX. Please note that for manual staining we recommend to optimize the primary antibody concentration and incubation time (overnight incubation), and amplification may be require
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (9)
ab53143 は 9 報の論文で使用されています。
- Wang Y et al. MicroRNA-125b as a tumor suppressor by targeting MMP11 in breast cancer. Thorac Cancer 11:1613-1620 (2020). PubMed: 32291953
- Layton TB et al. Cellular census of human fibrosis defines functionally distinct stromal cell types and states. Nat Commun 11:2768 (2020). PubMed: 32488016
- Jiang J et al. Identification of Aurora Kinase A as a Biomarker for Prognosis in Obesity Patients with Early Breast Cancer. Onco Targets Ther 13:4971-4985 (2020). PubMed: 32581556
- Liu S et al. Circ_0000260 Regulates the Development and Deterioration of Gastric Adenocarcinoma with Cisplatin Resistance by Upregulating MMP11 via Targeting MiR-129-5p. Cancer Manag Res 12:10505-10519 (2020). PubMed: 33122949
- Yang Y et al. LncRNA CTBP1-AS2 Facilitates Gastric Cancer Progression via Regulating the miR-139-3p/MMP11 Axis. Onco Targets Ther 13:11537-11547 (2020). PubMed: 33204108
- Lai Z et al. Circular RNA 0047905 acts as a sponge for microRNA4516 and microRNA1227-5p, initiating gastric cancer progression. Cell Cycle 18:1560-1572 (2019). PubMed: 31157588
- Niwa T et al. The dynamics of TGF-ß in dental pulp, odontoblasts and dentin. Sci Rep 8:4450 (2018). WB . PubMed: 29535349
- Jiang R et al. Mixomics analysis of breast cancer: Long non-coding RNA linc01561 acts as ceRNA involved in the progression of breast cancer. Int J Biochem Cell Biol 102:1-9 (2018). WB . PubMed: 29890225
- Roscilli G et al. Circulating MMP11 and specific antibody immune response in breast and prostate cancer patients. J Transl Med 12:54 (2014). ELISA ; Human . PubMed: 24564996