Application
Western blot
Sample
Human Cell lysate - whole cell (endothelial cells)
Loading amount
5000 cells
Specification
endothelial cells
Gel Running Conditions
Reduced Denaturing (10% acrylamide gel)
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 21°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C
Secondary antibody
Name
Non-Abcam antibody was used: Goat anti Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
Immobilon
Exposure
1 minute(s) and 30 second(s)
Bands
Specific: 63 kDa Non-specific: 100, 48, 35 kDa
Positive control
The 'non-specific' may be pro-forms or cleaved versions of the peptide?
Additional data
Abcam response
Our Scientific Support team is currently investigating this negative Abreview, and we will resolve the issue in accordance with our Abpromise.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Mar 01 2013