MMP-3 Activity Assay Kit (Fluorometric) (ab118972)
Key features and details
- Assay type: Enzyme activity
- Detection method: Fluorescent
- Platform: Microplate reader
- Assay time: 1 hr
- Sample type: Cell culture media, Cell culture supernatant, Milk, Tissue Extracts, Urine
- Sensitivity: 0.05 mU/well
製品の概要
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製品名
MMP-3 Activity Assay Kit (Fluorometric)
MMP3 キット 製品一覧 -
検出方法
Fluorescent -
サンプルの種類
Cell culture supernatant, Milk, Urine, Tissue Extracts, Cell culture media -
アッセイタイプ
Enzyme activity -
検出感度
< 0.05 mU/well -
全工程の試験時間
1h 00m -
種交差性
交差種: Mammals, Other species -
製品の概要
In Abcam's MMP-3 Activity Assay Kit, MMP-3 hydrolyzes a specific FRET substrate to release the quenched fluorescent group Mca, which can be detected fluorometrically at Ex/Em=325/393 nm. The kit provides a rapid, simple, sensitive and reliable test which can also be used as a high throughput assay of MMP-3 activity. The assay sensitivity is < 50 µU.
Visit our FAQs page for tips and troubleshooting.
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特記事項
This product is manufactured by BioVision, an Abcam company and was previously called K783 MMP-3 Activity Fluorometric Assay Kit. K783-100 is the same size as the 100 test size of ab118972.
The matrix metalloproteinase-3 (MMP-3, stromelysin-1) exhibits a number of activities that would make it a particularly good tumor promoter. Like several other MMPs, MMP-3 was first cloned and later recloned as a cancer-specific gene. In addition to degrading numerous extracellular matrix components, MMP-3 can activate gelatinase B, the collagenases and several serpin-type serine proteinase inhibitors. Moreover, it can release a number of cell surface molecules, including E-cadherin, a known contributor to cancer development.
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試験プラットフォーム
Microplate reader
製品の特性
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保存方法
Store at -20°C. Please refer to protocols. -
内容 100 tests MCA Peptide Standard 1 x 20µl MMP-1/3 Assay Buffer 1 x 25ml MMP-3 Positive Control 1 vial MMP-3 Substrate 1 x 200µl -
研究分野
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機能
Can degrade fibronectin, laminin, gelatins of type I, III, IV, and V; collagens III, IV, X, and IX, and cartilage proteoglycans. Activates procollagenase. -
配列類似性
Belongs to the peptidase M10A family.
Contains 4 hemopexin-like domains. -
ドメイン
The conserved cysteine present in the cysteine-switch motif binds the catalytic zinc ion, thus inhibiting the enzyme. The dissociation of the cysteine from the zinc ion upon the activation-peptide release activates the enzyme. -
細胞内局在
Secreted > extracellular space > extracellular matrix. - Information by UniProt
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別名
- CHDS6
- Matrix metalloproteinase 3
- Matrix metalloproteinase 3 preproprotein
see all
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (10)
ab118972 は 10 報の論文で使用されています。
- Cheng P et al. Smad3 regulates smooth muscle cell fate and mediates adverse remodeling and calcification of the atherosclerotic plaque. Nat Cardiovasc Res 1:322-333 (2022). PubMed: 36246779
- Deng Y et al. Estradiol/GPER affects the integrity of mammary duct-like structures in vitro. Sci Rep 10:1386 (2020). PubMed: 31992771
- Xiao S et al. Kruppel-like factor 2 disturb non-small cell lung cancer energy metabolism by inhibited glutamine consumption. J Pharm Pharmacol 72:843-851 (2020). PubMed: 32196690
- Hsiao YH et al. Phloretin suppresses metastasis by targeting protease and inhibits cancer stemness and angiogenesis in human cervical cancer cells. Phytomedicine 62:152964 (2019). PubMed: 31153059
- Hadi T et al. Macrophage-derived netrin-1 promotes abdominal aortic aneurysm formation by activating MMP3 in vascular smooth muscle cells. Nat Commun 9:5022 (2018). PubMed: 30479344
- Choi J et al. Interplay between RNASEH2 and MOV10 controls LINE-1 retrotransposition. Nucleic Acids Res 46:1912-1926 (2018). PubMed: 29315404
- O'Callaghan J et al. Therapeutic potential of AAV-mediated MMP-3 secretion from corneal endothelium in treating glaucoma. Hum Mol Genet 26:1230-1246 (2017). PubMed: 28158775
- Pettersson F et al. Genetic and pharmacologic inhibition of eIF4E reduces breast cancer cell migration, invasion, and metastasis. Cancer Res 75:1102-12 (2015). PubMed: 25608710
- Sun H et al. Hyperglycemia-suppressed expression of Serpine1 contributes to delayed epithelial wound healing in diabetic mouse corneas. Invest Ophthalmol Vis Sci 56:3383-92 (2015). PubMed: 26024123
- Turturro S et al. Sustained release of matrix metalloproteinase-3 to trabecular meshwork cells using biodegradable PLGA microparticles. Mol Pharm 10:3023-3032 (2013). PubMed: 23795867