Application
Western blot
Sample
Mouse Tissue lysate - other (Nuclei and mitochindria isolated from mice livers)
Loading amount
20 µg
Specification
Nuclei and mitochindria isolated from mice livers
Gel Running Conditions
Reduced Denaturing (8% gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: PBSx1 + 20% tween 20, 5% BSA, 0.02%AZID
Secondary antibody
Name
Non-Abcam antibody was used: Peroxidase conjugated affunipure Goat anti Rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
2 minute(s) and 0 second(s)
Bands
Specific: 95 kDa Non-specific: 72, 45, 110, 160 kDa
Positive control
extracts from isolated mitochondria vrs extracts from isolated nuclei
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投稿 Aug 08 2012