Anti-MKRN1 抗体 (ab123804)
Key features and details
- Goat polyclonal to MKRN1
- Suitable for: WB
- Reacts with: Mouse, Human
- Isotype: IgG
製品の概要
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製品名
Anti-MKRN1 antibody
MKRN1 一次抗体 製品一覧 -
製品の詳細
Goat polyclonal to MKRN1 -
由来種
Goat -
特異性
ab123804 is expected to recognize both reported isoforms (NP_038474.2; NP_001138597.1). -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Mouse, Human
交差が予測される動物種: Cow, Dog, Pig -
免疫原
Synthetic peptide:
RYEHSKPLKQEEAT
by a Cysteine residue linker, corresponding to internal sequence amino acids 105-118 of Human MKRN1 (NP_038474.2; NP_001138597.1). -
ポジティブ・コントロール
- MKRN1 transfected HEK293 cells.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
pH: 7.3
Preservative: 0.02% Sodium azide
Constituents: 0.5% BSA, 99% Tris buffered saline -
Concentration information loading...
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精製度
Immunogen affinity purified -
特記事項(精製)
ab123804 was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab123804の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa).
1 hour primary incubation is recommended for this product. |
特記事項 |
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WB
Use a concentration of 0.3 - 1 µg/ml. Detects a band of approximately 60 kDa (predicted molecular weight: 53 kDa). 1 hour primary incubation is recommended for this product. |
ターゲット情報
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機能
E3 ubiquitin ligase catalyzing the covalent attachment of ubiquitin moieties onto substrate proteins. These substrates include FILIP1, p53/TP53, CDKN1A and TERT. Keeps cells alive by suppressing p53/TP53 under normal conditions, but stimulates apoptosis by repressing CDKN1A under stress conditions. Acts as a negative regulator of telomerase. Has negative and positive effects on RNA polymerase II-dependent transcription. -
組織特異性
Ubiquitous. -
パスウェイ
Protein modification; protein ubiquitination. -
配列類似性
Contains 4 C3H1-type zinc fingers.
Contains 1 RING-type zinc finger. -
翻訳後修飾
Auto-ubiquitinated; which leads to proteasomal degradation. - Information by UniProt
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参照データベース
- Entrez Gene: 514986 Cow
- Entrez Gene: 475528 Dog
- Entrez Gene: 23608 Human
- Entrez Gene: 54484 Mouse
- Entrez Gene: 100514261 Pig
- Omim: 607754 Human
- SwissProt: Q9UHC7 Human
- SwissProt: Q9QXP6 Mouse
see all -
別名
- E3 ubiquitin-protein ligase makorin-1 antibody
- FLJ21334 antibody
- HGNC:7112 antibody
see all
画像
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All lanes : Anti-MKRN1 antibody (ab123804) at 1 µg/ml
Lane 1 : fetal mouse brain tissue lysate
Lane 2 : adult mouse brain tissue lysate
Lysates/proteins at 35 µg per lane.
Predicted band size: 53 kDa
Observed band size: 50 kDa why is the actual band size different from the predicted?
Additional bands at: 35 kDa (possible isoform) -
Lane 1 : Anti-MKRN1 antibody (ab123804) at 0.3 µg/ml
Lane 2 : Anti-MKRN1 antibody (ab123804) at 1 mg/ml
Lane 3 : anti-DYKDDDDK Tag anntibody at 1/1000 dilution
Lane 1 : HEK293 lysate, over-expressing Human MKRN1 with DYKDDDDK tag in RIPA buffer
Lane 2 : HEK293 lysate, mock-transfected
Lane 3 : HEK293 lysate, over-expressing Human MKRN1 with DYKDDDDK tag in RIPA buffer
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Predicted band size: 53 kDa
Observed band size: 60 kDa why is the actual band size different from the predicted?
データシートおよび資料
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Datasheet download
参考文献 (0)
ab123804 は論文での使用が確認できていません。