Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Tonsil (periphery))
Specification
Tonsil (periphery)
Fixative
Formaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: citrate buffer
Permeabilization
No
Blocking step
(agent) for 5 minute(s) · Concentration: 100% · Temperature: 25°C
Other product details
Dilution
1/100
Incubation time
30 minute(s) · Temperature: 25°C · Diluent: diluent from another company
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit IgG
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Host species: Goat
Clonality: Polyclonal
Conjugation: Biotin
Dilution
1/500
Additional data
Additional Notes
good staining of endothelium & vascular smooth muscle
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. R Berahovich
Verified customer
投稿 Dec 01 2011