Application
Western blot
Sample
Mouse Tissue lysate - whole (liver)
Loading amount
30 µg
Specification
liver
Gel Running Conditions
Reduced Denaturing (10)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Other product details
Dilution
1/2000
Incubation time
1 hour(s) and 0 minute(s) · Diluent: 5% Milk in TBST
Secondary antibody
Name
Abcam antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)
Dilution
1/5000
Detection
Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 13 kDa
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Jan 10 2011