Anti-MIF 抗体 (ab65869)
Key features and details
- Rabbit polyclonal to MIF
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
リコンビナント抗体で、ロット間での高い再現性を実現
- 異なるロット間での安定した再現性
- 容易なスケールアップ
- 評価試験による特異性の確認済み
- 倫理基準に準拠 - アニマル・フリーの生産
製品の概要
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製品名
Anti-MIF antibody
MIF 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MIF -
由来種
Rabbit -
アプリケーション
適用あり: WB, IHC-Pmore details -
種交差性
交差種: Mouse, Rat, Human -
免疫原
Synthetic peptide corresponding to Human MIF (C terminal). A synthetic peptide corresponding to a sequence at the C-terminus of human MIF, different from the related rat and mouse sequences by one amino acid.
Database link: P14174 -
ポジティブ・コントロール
- IHC-P: Rat kidney and liver tissue, mammary cancer tissue lysate. WB: HeLa, Colo320, MM231 cell lysates.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Add 0.2ml of distilled water to yield a dilution of 500 ug/mL -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab65869の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB | (2) |
Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa).
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IHC-P | (1) |
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
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特記事項 |
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WB
Use a concentration of 1 µg/ml. Detects a band of approximately 13 kDa (predicted molecular weight: 13 kDa). |
IHC-P
Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. |
ターゲット情報
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機能
Pro-inflammatory cytokine. Involved in the innate immune response to bacterial pathogens. The expression of MIF at sites of inflammation suggests a role as mediator in regulating the function of macrophages in host defense. Counteracts the anti-inflammatory activity of glucocorticoids. Has phenylpyruvate tautomerase and dopachrome tautomerase activity (in vitro), but the physiological substrate is not known. It is not clear whether the tautomerase activity has any physiological relevance, and whether it is important for cytokine activity. -
関連疾患
Genetic variations in MIF are associated with susceptibility to rheumatoid arthritis systemic juvenile (RASJ) [MIM:604302]. An inflammatory articular disorder with systemic-onset beginning before the age of 16. It represents a subgroup of juvenile arthritis associated with severe extraarticular features and occasionally fatal complications. During active phases of the disorder, patients display a typical daily spiking fever, an evanescent macular rash, lymphadenopathy, hepatosplenomegaly, serositis, myalgia and arthritis. -
配列類似性
Belongs to the MIF family. -
細胞内局在
Secreted. Cytoplasm. Does not have a cleavable signal sequence and is secreted via a specialized, non-classical pathway. Secreted by macrophages upon stimulation by bacterial lipopolysaccharide (LPS), or by M.tuberculosis antigens. - Information by UniProt
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参照データベース
- Entrez Gene: 4282 Human
- Entrez Gene: 17319 Mouse
- Entrez Gene: 81683 Rat
- Omim: 153620 Human
- SwissProt: P14174 Human
- SwissProt: P34884 Mouse
- SwissProt: P30904 Rat
- Unigene: 407995 Human
see all -
別名
- GIF antibody
- GLIF antibody
- Glycosylation inhibiting factor antibody
see all
画像
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MIF was detected in paraffin-embedded section of human mammary cnacer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65869 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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MIF was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml ab65869 overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex with DAB as the chromogen.
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All lanes : Anti-MIF antibody (ab65869)
Lane 1 : HeLa cell lysate
Lane 2 : COLO320 cell lysate
Lane 3 : MM231 cell lysate
Predicted band size: 13 kDa
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (5)
ab65869 は 5 報の論文で使用されています。
- Song X et al. Construction of a Novel Ferroptosis-Related Gene Signature for Predicting Survival of Patients With Lung Adenocarcinoma. Front Oncol 12:810526 (2022). PubMed: 35311093
- Xue N et al. Plasma Macrophage Migration Inhibitory Factor and CCL3 as Potential Biomarkers for Distinguishing Patients with Nasopharyngeal Carcinoma from High-Risk Individuals Who Have Positive Epstein-Barr Virus Capsid Antigen-Specific IgA. Cancer Res Treat 51:378-390 (2019). PubMed: 29807404
- Abu El-Asrar AM et al. The Proinflammatory and Proangiogenic Macrophage Migration Inhibitory Factor Is a Potential Regulator in Proliferative Diabetic Retinopathy. Front Immunol 10:2752 (2019). PubMed: 31866994
- Li S et al. Macrophage Migration Inhibitory Factor Mediates Neuroprotective Effects by Regulating Inflammation, Apoptosis and Autophagy in Parkinson's Disease. Neuroscience 416:50-62 (2019). PubMed: 31170483
- Guo F et al. Identification of differentially expressed inflammatory factors in Wilms tumors and their association with patient outcomes. Oncol Lett 14:687-694 (2017). PubMed: 28693222