Application
Western blot
Sample
Human Cell lysate - whole cell (cell lines)
Loading amount
20 µg
Specification
cell lines
Gel Running Conditions
Reduced Denaturing (4-15% Tris-glycine gel)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Dilution
1/200
Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% BSA in Tween-PBS
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/10000
Detection
Detection method
ECL
Exposure
30 minute(s) and 0 second(s)
Bands
Specific: 180 kDa Non-specific: 48 kDa
Positive control
A172 cells
Negative control
CV1 cells, HeLa cells
Additional data
Additional Notes
works great. one non-specific band at 48 kDa
Abcam response
We would like to thank the reviewer for submitting this Abreview for ab52968. We are currently unsure as to the identity of the band at 48kDA.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Mr. Nir Drayman
Verified customer
投稿 Mar 05 2012