PE Anti-MEKK2 抗体 [EP626Y] (ab210719)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- PE Rabbit monoclonal [EP626Y] to MEKK2
- Suitable for: Flow Cyt (Intra), ICC/IF
- Reacts with: Human
- Conjugation: PE. Ex: 488nm, Em: 575nm
Related conjugates and formulations
製品の概要
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製品名
PE Anti-MEKK2 antibody [EP626Y]
MEKK2 一次抗体 製品一覧 -
製品の詳細
PE Rabbit monoclonal [EP626Y] to MEKK2 -
由来種
Rabbit -
標識
PE. Ex: 488nm, Em: 575nm -
アプリケーション
適用あり: Flow Cyt (Intra), ICC/IFmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat -
免疫原
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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ポジティブ・コントロール
- Flow Cyt (intra)ometry: Jurkat cells ICC/IF: Jurkat cells
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特記事項
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark. -
バッファー
pH: 7.4
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS -
Concentration information loading...
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精製度
Protein A purified -
ポリ/モノ
モノクローナル -
クローン名
EP626Y -
アイソタイプ
IgG -
研究分野
関連製品
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Alternative Versions
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Isotype control
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Positive Controls
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab210719の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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Flow Cyt (Intra) |
1/1000.
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ICC/IF |
1/100.
This product gave a positive signal in Jurkat cells fixed with 4% formaldehyde, and 80% methanol (5 min). |
特記事項 |
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Flow Cyt (Intra)
1/1000. |
ICC/IF
1/100. This product gave a positive signal in Jurkat cells fixed with 4% formaldehyde, and 80% methanol (5 min). |
ターゲット情報
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機能
Component of a protein kinase signal transduction cascade. Regulates the JNK and ERK5 pathways by phosphorylating and activating MAP2K5 and MAP2K7 (By similarity). Plays a role in caveolae kiss-and-run dynamics. -
配列類似性
Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. MAP kinase kinase kinase subfamily.
Contains 1 OPR domain.
Contains 1 protein kinase domain. -
翻訳後修飾
Autophosphorylated. -
細胞内局在
Cytoplasm. Nucleus. Upon EGF stimulation, translocates into the nucleus. - Information by UniProt
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参照データベース
- Entrez Gene: 10746 Human
- Entrez Gene: 26405 Mouse
- Entrez Gene: 171492 Rat
- Omim: 609487 Human
- SwissProt: Q9Y2U5 Human
- SwissProt: Q61083 Mouse
- Unigene: 145605 Human
- Unigene: 211762 Mouse
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別名
- M3K2_HUMAN antibody
- Map3k2 antibody
- MAPK/ERK kinase kinase 2 antibody
see all
画像
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ab210719 staining MEKK2 in Jurkat cells. The cells were fixed with 4% formaldehyde, permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1%PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab210719 at 1/100 dilution (pseudocolored in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
This product also gave a positive signal under the same testing conditions in Jurkat cells fixed with 80%methanol.
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Overlay histogram showing Jurkat cells stained with ab210719 (red line). The cells were fixed with 4% formaldehyde and then permeabilized with 90% methanol at -20°C for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab210719, 1/1000 dilution) for 30 min at 22°C.
Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control.
Acquisition of >5,000 events were collected using a 50mW Yellow/Green laser (561nm) and 586/15 bandpass filter.
データシートおよび資料
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SDS download
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Datasheet download
Certificate of Compliance
参考文献 (0)
ab210719 は論文での使用が確認できていません。