Application
Western blot
Sample
Human Cell lysate - whole cell (LnCap, PC3, HeLa cells)
Gel Running Conditions
Non-reduced Denaturing
Loading amount
10 µg
Specification
LnCap, PC3, HeLa cells
Blocking step
Sigma-AldrichWestern Blocker Solution (W0138) as blocking agent for 12 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 4°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: Sigma Western Blocker Solution (W0138)
Dilution
1/1000
Secondary antibody
Dilution
1/30000
Name
Non-Abcam antibody was used: Goat anti-Rabbit IgG (whole molecule)–Peroxidase a
Host species: Goat
Clonality: Monoclonal
Conjugation: HRP polymer
Host species: Goat
Clonality: Monoclonal
Conjugation: HRP polymer
Detection
Detection method
ECL
Exposure
5 second(s)
Bands
Specific: 44 kDa Non-specific: 37, 34, kDa
Additional data
Additional Notes
The attached ECL exposure shows three lanes of 10ug protein each. Lane 1 is LnCap cell lysate lane 2 is PC3 cell lysate lane 3 is HeLa cell lysate. LnCap and PC3 are human prostate cancer cell lines. The upper band is ~44 kDa, the lower is ~37 kDa, then 34 kDa, then several bands below 30 kDa.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Nov 01 2007