Application
Western blot
Sample
Rat Tissue lysate - whole (liver)
Loading amount
30 µg
Specification
liver
Treatment
2mg/kg LPS 1h
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C
Other product details
Dilution
1/2000
Incubation time
12 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 5% milk, 0,5% tween 20
Secondary antibody
Name
Abcam antibody:Goat polyclonal Secondary Antibody to Rabbit IgG - H&L (HRP)
Dilution
1/5000
Detection
Detection method
ECL
Exposure
10 minute(s) and 0 second(s)
Bands
Specific: 17-19 kDa Non-specific: 14 kDa
Positive control
line 1: LPS 2mg/kg 1h
Negative control
line 3: untreated rats
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Nov 16 2012