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Western blot - MCM7 antibody (ab51062)
Western blot - MCM7 antibody (ab51062)
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Product Name 

MCM7 antibody

 

MCM7 抗体 (8件) 一覧

Product type 

Primary antibodies

Description 

Rabbit polyclonal to MCM7

Immunogen 

Synthetic peptide corresponding to residues near the N-terminus of human MCM7.

Reacts with 

Hu, Rat

Does not react with 
(species key)

Ms

Tested applications 
(see key)

IP, WB


Abreviews 

Customer reviews
(see key)


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Application notes 
(see key)

Recommended dilutions
IP: 1/20.
WB: 1/5000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).

Is unsuitable for ICC/IF or IHC.

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

HeLa cell lysate.

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other

Relevance 

hCDC47 is a human member of the MCM family, which allows DNA to replicate once per cell cycle. In quiescent cells, human MCM7 (hMCM7) mRNA is almost undetectable. Stimulation of cells to enter the cell cycle results in induction of hMCM7 expression. The hCDC47 protein expression and localization is found in nuclei of the proliferative components of normal lymph nodes, bone marrow, epidermis and mucosa. Malignant tumors from several organs contained more nuclear hCDC47 positive cells than their normal counterparts. These results indicate that hCDC47 may play a role in normal and neoplastic cell growth in vivo.

 

MCM7 antibody (ab51062)の別名

Database links 

The links below go to external sites and will open in a new browser window

Entrez Gene

    

4176    (Human)
288532    (Rat)

Omim

    

600592    (Human)

SwissProt

    

P33993    (Human)
Q6AYN8    (Rat)

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Protein A purified

Storage buffer 

Preservative: 0.25% Sodium Azide
Constituents: 40% Glycerol, 0.5% BSA, 0.18M Sodium chloride, 50mM Tris glycine, 25mM Citric acid, 0.25mM EDTA, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

この製品は、毒物及び劇物取締法に基づく「毒物」に該当します。

Form 

Liquid

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

 

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See below for MCM7 antibody images, references, products related to ab51062 and other tools.

 

MCM7 antibody images:

Product image  Western blot - MCM7 antibody (ab51062)

Western blot - MCM7 antibody (ab51062)

MCM7 antibody (ab51062) at 1/5000 dilution + HeLa cell lysate at 10 µg

Secondary
Goat anti-Rabbit HRP labeled. at 1/2000 dilution

Predicted band size : 81 kDa
Observed band size : 81 kDa

 
 

Search PubMed (MEDLINE) for references to MCM7

 

MCM7 antibody - more information

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Price and availability of products related to MCM7 antibody

 

MCM7 antibody - related products:

Products for Western blot

Loading controls

Lysates

 

Positive Controls for MCM7 antibody

ab14655

  

HeLa (Human epithelial carcinoma cell line) Nuclear Lysate

 

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MCM7 antibody - other tools:

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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"