Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Overview
Product name
Anti-MCM7 antibody
MCM7 抗体 (10件) 一覧
Description
Goat polyclonal to MCM7
Tested applications
Cross reactivity
Reacts with
Human
Predicted to work with
Mouse
Immunogen
Synthetic peptide (Human) - which represented a portion of human Minichromosome Maintenance Deficient 7 encoded in part by exons 8 and 9.
Positive control
HeLa cell lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 8mM PBS, 60mM Citrate, 150mM Tris, pH 7-8
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
-
Western blot - MCM7 antibody (ab3732)
(enlarge)
Applications
Show applications key
Our Abpromise guarantee covers the use of ab3732 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: 1/2000 - 1/10000.Detects a band of approximately 90 kDa (predicted molecular weight: 90 kDa).
Target
Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for S-phase checkpoint activation upon UV-induced damage.
Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain.
Post-translational
modifications
Phosphorylated upon DNA damage, probably by ATM or ATR.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP33993
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- CDABP0042 antibody
- CDC 47 antibody
- CDC47 antibody
see all
Database links
- Entrez Gene: 4176 Human
- Entrez Gene: 17220 Mouse
- Omim: 600592 Human
- SwissProt: P33993 Human
- SwissProt: Q3V122 Mouse
- SwissProt: Q61881 Mouse
- Unigene: 438720 Human
- Unigene: 378965 Mouse
Anti-MCM7 antibody images:
Western blot - MCM7 antibody (ab3732)
Predicted band size : 90 kDa
Sample: Hela cell RIPA extract (60
Antibody: Affinity purified Goat anti-MCM 7 (an3732) diluted 1 / 4000.
Detection: ECL.
References for Anti-MCM7 antibody (ab3732)
ab3732 has not yet been referenced specifically in any publications.
Publishing research using ab3732? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"