MCM7 antibody [EP1974Y] (ab52489)

Product Name 

MCM7 antibody [EP1974Y]

Product type 

Primary antibodies

Description 

Rabbit monoclonal [EP1974Y] to MCM7

Immunogen 

Synthetic peptide corresponding to residues near the C terminus of human MCM7

Reacts with 
(species key)

Hu, Ms, Rat

Specificity 

This antibody reacts with hCDC47

Tested applications 
(see key)

Flow Cyt, ICC, IHC-P, IP, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews

Application notes 
(see key)

Recommended dilutions
Flow Cyt: 1/40.
ICC: 1/100 - 1/250.
IHC-P: 1/100 - 1/250. It is strongly recommended to perform an antigen retrieval step for an enhanced signal.
IP: 1/40.
WB: 1/10,000. Detects a band of approximately 81 kDa (predicted molecular weight: 81 kDa).

Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

Human lung tissue and Hela cell lysate

Cellular localization 

Nuclear

Research areas 

Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other

Relevance 

hCDC47 is a human member of the MCM family, which allows DNA to replicate once per cell cycle. In quiescent cells, human MCM7 (hMCM7) mRNA is almost undetectable. Stimulation of cells to enter the cell cycle results in induction of hMCM7 expression. The hCDC47 protein expression and localization is found in nuclei of the proliferative components of normal lymph nodes, bone marrow, epidermis and mucosa. Malignant tumors from several organs contained more nuclear hCDC47 positive cells than their normal counterparts. These results indicate that hCDC47 may play a role in normal and neoplastic cell growth in vivo.

Database links 

Raised in 

Rabbit

Clonality 

Monoclonal

Clone number 

EP1974Y

Isotype 

IgG

Purity 

Tissue culture supernatant

Storage buffer 

Preservative: 0.01% Sodium Azide
Constituents: 40% Glycerol, 0.05% BSA, Tissue culture supernatant, 0.15M Sodium chloride, 50mM Tris glycine, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

Concentration not determined - その理由

Storage instructions 

Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

Notes 

Produced under U.S. Patent No. 5,675,063.

See below for MCM7 antibody [EP1974Y] images, references, products related to ab52489 and other tools.

MCM7 antibody [EP1974Y] images:

References for MCM7 antibody [EP1974Y] (ab52489)

  This product has been used in: (two most recent references)

Takahashi N et al. The MCM-binding protein ETG1 aids sister chromatid cohesion required for postreplicative homologous recombination repair. PLoS Genet 6:e1000817 (2010). WB; Human. PubMed: 20090939

Xu X et al. MCM10 mediates RECQ4 association with MCM2-7 helicase complex during DNA replication. EMBO J 28:3005-14 (2009). Human. PubMed: 19696745
If you publish research using ab52489 please let us know so that we can cite the reference on this datasheet.

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