Anti-MCM7 antibody [EP1974Y] (ab52489)

  Western blot - MCM7 antibody [EP1974Y] (ab52489)

Anti-MCM7 antibody [EP1974Y] (ab52489) at 1/10000 dilution + Hela cell lysate at 10 µg

Secondary
Goat anti-rabbit HRP labeled at 1/2000 dilution

Predicted band size : 81 kDa
Observed band size : 81 kDa

  Immunohistochemistry (Paraffin-embedded sections) - MCM7 antibody [EP1974Y] (ab52489)

Immunohistochemical analysis of paraffin-embedded human lung tissue using ab52489 at a 1/100 dilution.

  Immunocytochemistry/ Immunofluorescence - MCM7 antibody [EP1974Y] (ab52489)

ICC/IF image of ab52489 stained MCF7 cells. The cells were 4% PFA fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2489, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

  Flow Cytometry - MCM7 antibody [EP1974Y] (ab52489)

Overlay histogram showing HeLa cells stained with ab52489 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab52489, 1/100 dilution) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-rabbit IgG (H+L) (ab96899) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) (1μg/1x10⁶ cells) used under the same conditions. Acquisition of >5,000 events was performed.