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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM7 antibody [47DC141] (ab2360)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM7 antibody [47DC141] (ab2360)](/ps/datasheet/images/ab2360_1.jpg)
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Immunocytochemistry/ Immunofluorescence - MCM7 antibody [47DC141] (ab2360)
![Immunocytochemistry/ Immunofluorescence - MCM7 antibody [47DC141] (ab2360)](/ps/datasheet/images/ab2360_2.jpg)
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Western blot - MCM7 antibody [47DC141] (ab2360)
![Western blot - MCM7 antibody [47DC141] (ab2360)](/ps/datasheet/images/ab2360_3a.jpg)
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Product Name
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MCM7 antibody [47DC141]
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MCM7 抗体 (8件) 一覧
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [47DC141] to MCM7
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Immunogen
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Recombinant full length protein (Human).
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Reacts with
(species key)
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Hu, Ms, Rat, Dog, Xl
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Tested applications
(see key)
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ICC/IF, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ICC/IF: Use at a concentration of 1 µg/ml.
IF: Use at an assay dependent dilution.
IHC-P: 1/50 - 1/100. This is when using an ABC method for 30 minutes at room temperature. Sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
IP: Use at 2µg/mg of lysate.
WB: 1/25 - 1/50. Incubate for 2 hrs at RT for colorimetric detection, can dilute more with ECL+ and with overnight incubation.
By Western blot, this antibody detects a band of 80 kDa, which corresponds to the predicted molecular weight of Cdc47 / MCM7. Detects a band of approximately 80 kDa (predicted molecular weight: 80 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Breast carcinoma, MAD109 cell lysate, PC12 cell lysate.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
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Relevance
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hCDC47 is a human member of the MCM family, which allows DNA to replicate once per cell cycle. In quiescent cells, human MCM7 (hMCM7) mRNA is almost undetectable. Stimulation of cells to enter the cell cycle results in induction of hMCM7 expression. The hCDC47 protein expression and localization is found in nuclei of the proliferative components of normal lymph nodes, bone marrow, epidermis and mucosa. Malignant tumors from several organs contained more nuclear hCDC47 positive cells than their normal counterparts. These results indicate that hCDC47 may play a role in normal and neoplastic cell growth in vivo.
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MCM7 antibody [47DC141] (ab2360)の別名 |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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47DC141
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Isotype
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IgG1
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Purity
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IgG fraction
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 1% BSA Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.100 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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Abcam では全ての製品情報を一元管理しており、 MCM7 antibody [47DC141] について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。 |
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See below for MCM7 antibody [47DC141] images, references, products related to ab2360 and other tools.
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MCM7 antibody [47DC141] images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM7 antibody [47DC141] (ab2360)
ab2360 - immunohistochemistry
Formalin fixed paraffin embedded human tonsil stained with MCM7, using ABC and DAB chromagen.
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Immunocytochemistry/ Immunofluorescence - MCM7 antibody [47DC141] (ab2360)
This image shows immunostaining of rat brain endothelial cells. Brain endothelial cells were co-cultured with neuronal precursor cells and the nuclear staining represents cells in cell cycle. Primary antibody (ab2360) was used at 1:50 dilution, incubated overnight at 4 oC. Secondary antibody - Alexafluor (488 nm) at 1:200 dilution, incubated for 2 hours at room temperature.
The picture was kindly supplied by Dr Joseph Corteza Lim and Dr Margery Barrand from University of Cambridge, Department of Pharmacology.
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Western blot - MCM7 antibody [47DC141] (ab2360)
All lanes : MCM7 antibody [47DC141] (ab2360) at 1/200 dilution
Lane 1 : M phase Xenopus laevis egg extract, whole tissue lysate.
Lane 2 : I phase Xenopus laevis egg extract, whole tissue lysate.
Secondary
HRP conjugated Donkey anti-rabbit IgG
developed using the ECL technique
Predicted band size : 80 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute This image is courtesy of an anonymous Abreview
See Abreview
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM7 antibody [47DC141] (ab2360)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM7 antibody [47DC141] (ab2360)](/ps/datasheet/images/2/ab2360/MCM7-Primary-antibodies-ab2360-5.jpg)
ab2360 staining MCM7 in human bladder cancer tissue sections by Immunohistochemistry (formalin fixed sections). Tissue was fixed with formaldehyde and a heat mediated antigen retrieval step was performed using citrate buffer. Tissue was blocked with 5% BSA for 1 hour at room temperature followed by incubation with the primary antibody at a 1/1200 dilution for 1 hour. A HRP-conjugated goat anti-mouse polyclonal was used as secondary antibody un-diluted. Image kindly supplied by Dr Karin Birkenkamp-Demtroeder through Abreview
See Abreview
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Western blot - MCM7 antibody [47DC141] (ab2360)
![Western blot - MCM7 antibody [47DC141] (ab2360)](/ps/datasheet/images/2/ab2360/MCM7-Primary-antibodies-ab2360-6.jpg)
Lane 1 : MCM7 antibody [47DC141] (ab2360) at 1/50 dilution
Lane 2 : MCM7 antibody [47DC141] (ab2360) at 1/200 dilution
Lane 3 : MCM7 antibody [47DC141] (ab2360) at 1/500 dilution
Lane 1 : Whole cell lysate prepared from SW780 cells
Lane 2 : Whole cell lysate prepared from SW780 cells
Lane 3 : Whole cell lysate prepared from SW780 cells
Lysates/proteins at 25 µg per lane.
Secondary
Goat anti-mouse IgG conjugated to HRP at 1/5000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 80 kDa
Observed band size : 81 kDa (why is the actual band size different from the predicted?)
Exposure time : 10 minutes
Gel run under denaturing conditions 4-12% gradient. Image kindly supplied by Dr Karin Birkenkamp-Demtroeder through Abreview
See Abreview
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Immunocytochemistry/ Immunofluorescence-MCM7 antibody [47DC141](ab2360)
](\ps\datasheet\images\2\ab2360\MCM7-Primary-antibodies-ab2360-7.jpg)
ICC/IF image of ab2360 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab2360, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-mouse IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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References for MCM7 antibody [47DC141] (ab2360)
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This product has been used in: (two most recent references)
Pillaire MJ et al. A 'DNA replication' signature of progression and negative outcome in colorectal cancer. Oncogene 29:876-87 (2010). IF; Human. PubMed: 19901968
van Dekken H et al. Array comparative genomic hybridization, expression array, and protein analysis of critical regions on chromosome arms 1q, 7q, and 8p in adenocarcinomas of the gastroesophageal junction. Cancer Genet Cytogenet 189:37-42 (2009). PubMed: 19167610
See all 4 publication references for this product.
If you publish research using ab2360 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MCM7
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MCM7 antibody [47DC141] - more information
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MCM7 antibody [47DC141] - related products:
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Contact Abcam with a Technical Enquiry about MCM7 antibody [47DC141] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM7 antibody [47DC141] (ab2360)](/index.html?datasheet=2360#ab2360_1.jpg)
![Immunocytochemistry/ Immunofluorescence - MCM7 antibody [47DC141] (ab2360)](/index.html?datasheet=2360#ab2360_2.jpg)
![Western blot - MCM7 antibody [47DC141] (ab2360)](/index.html?datasheet=2360#ab2360_3a.jpg)
