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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM7 antibody [47DC141] (ab2360)
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM7 antibody [47DC141] (ab2360)](/ps/datasheet/images/ab2360_1.jpg)
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- MCM7 antibody [47DC141] (ab2360)
![- MCM7 antibody [47DC141] (ab2360)](/ps/datasheet/images/ab2360_2.jpg)
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Western blot - MCM7 antibody [47DC141] (ab2360)
![Western blot - MCM7 antibody [47DC141] (ab2360)](/ps/datasheet/images/ab2360_3a.jpg)
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Product Name
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MCM7 antibody [47DC141]
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MCM7 抗体 (7件) 一覧
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Product type
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Primary antibodies
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Description
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Mouse monoclonal [47DC141] to MCM7
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Immunogen
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Recombinant full length protein (Human).
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Reacts with
(species key)
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Hu, Ms, Rat, Dog, Xl
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Tested applications
(see key)
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ICC/IF, IHC-P, IP, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
WB: 1/25 - 1/50 for 2 hrs at RT for colorimetric detection, can dilute more with ECL+ and with overnight incubation.
By Western blot, this antibody detects a band of 80 kDa, which corresponds to the predicted molecular weight of Cdc47 / MCM7.
IP: Use at 2µg/mg of lysate.
IF: Use at an assay dependent dilution.
IHC-P: 1/50 - 1/100 in an ABC method for 30 minutes at room temperature. Sections require high temperature antigen unmasking with 10 mM citrate buffer, pH 6.0 prior to immunostaining.
Not tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Breast carcinoma, MAD109 cell lysate, PC12 cell lysate.
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Cellular localization
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Nuclear
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Research areas
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Chromatin and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
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Relevance
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hCDC47 is a human member of the MCM family, which allows DNA to replicate once per cell cycle. In quiescent cells, human MCM7 (hMCM7) mRNA is almost undetectable. Stimulation of cells to enter the cell cycle results in induction of hMCM7 expression. The hCDC47 protein expression and localization is found in nuclei of the proliferative components of normal lymph nodes, bone marrow, epidermis and mucosa. Malignant tumors from several organs contained more nuclear hCDC47 positive cells than their normal counterparts. These results indicate that hCDC47 may play a role in normal and neoplastic cell growth in vivo.
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MCM7 antibody [47DC141] (ab2360)の別名 |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Mouse
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Clonality
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Monoclonal
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Clone number
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47DC141
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Isotype
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IgG1
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Purity
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IgG fraction
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Storage buffer
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Preservative: 0.05% Sodium Azide
Constituents: 1% BSA Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Form
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Liquid
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Concentration
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0.100 mg/ml
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Storage instructions
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Store at +4°C. Do not freeze.
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Abcam では全ての製品情報を一元管理しており、 MCM7 antibody [47DC141] について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。 |
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See below for MCM7 antibody [47DC141] images, references, products related to ab2360 and other tools.
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MCM7 antibody [47DC141] images:
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 Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM7 antibody [47DC141] (ab2360)
ab2360 - immunohistochemistry
Formalin fixed paraffin embedded human tonsil stained with MCM7, using ABC and DAB chromagen.
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- MCM7 antibody [47DC141] (ab2360)
This image shows immunostaining of rat brain endothelial cells. Brain endothelial cells were co-cultured with neuronal precursor cells and the nuclear staining represents cells in cell cycle. Primary antibody (ab2360) was used at 1:50 dilution, incubated overnight at 4 oC. Secondary antibody - Alexafluor (488 nm) at 1:200 dilution, incubated for 2 hours at room temperature.
The picture was kindly supplied by Dr Joseph Corteza Lim and Dr Margery Barrand from University of Cambridge, Department of Pharmacology.
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Western blot - MCM7 antibody [47DC141] (ab2360)
All lanes : MCM7 antibody [47DC141] (ab2360) at 1/200 dilution
Lane 1 : M phase Xenopus laevis egg extract, whole tissue lysate.
Lane 2 : I phase Xenopus laevis egg extract, whole tissue lysate.
Secondary
HRP conjugated Donkey anti-rabbit IgG
developed using the ECL technique
Predicted band size : 80 kDa
Observed band size : 95 kDa (why is the actual band size different from the predicted?)
Exposure time : 1 minute This image is courtesy of an anonymous Abreview
See Abreview
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References for MCM7 antibody [47DC141] (ab2360)
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This product has been used in: (two most recent references)
Pillaire MJ et al. A 'DNA replication' signature of progression and negative outcome in colorectal cancer. Oncogene 29:876-87 (2010). IF; Human. PubMed: 19901968
van Dekken H et al. Array comparative genomic hybridization, expression array, and protein analysis of critical regions on chromosome arms 1q, 7q, and 8p in adenocarcinomas of the gastroesophageal junction. Cancer Genet Cytogenet 189:37-42 (2009). PubMed: 19167610
See all 4 publication references for this product.
If you publish research using ab2360 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MCM7
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MCM7 antibody [47DC141] - more information
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Customer reviews (feedback) regarding MCM7 antibody [47DC141] |
Customer FAQs regarding MCM7 antibody [47DC141] |
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MCM7 antibody [47DC141] - related products:
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MCM7 antibody [47DC141] - other tools:
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Contact Abcam with a Technical Enquiry about MCM7 antibody [47DC141] |
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All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Pricing information loading
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - MCM7 antibody [47DC141] (ab2360)](/index.html?datasheet=2360#ab2360_1.jpg)
![- MCM7 antibody [47DC141] (ab2360)](/index.html?datasheet=2360#ab2360_2.jpg)
![Western blot - MCM7 antibody [47DC141] (ab2360)](/index.html?datasheet=2360#ab2360_3a.jpg)
