Application
Western blot
Sample
Human Cell lysate - whole cell (HeLa)
Loading amount
50 µg
Specification
HeLa
Gel Running Conditions
Reduced Denaturing (12%)
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
5 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: blocking buffer
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-rabbit IgG HRP
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000
Detection
Detection method
ECL
Exposure
40 second(s)
Bands
Specific: 85 kDa
Positive control
First lane is Xenopus Laevis egg extract while second lane is HeLa cell extract.Xl-Mcm5 runs slightly larger than Hs-Mcm5.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 08 2011