Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
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Customer is using these two antibodies in Western blotting with HCT16 lysate. For both she is seeing a doublet at 125 kDa. |
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ANSWER: |
Thank you very much for your enquiry. The originator of these two antibodies has provided me with the following information. To their knowledge they have not heard of reports of doublets being seen with ab11897 and ab11896. Both of these were tested using Hela cell lysates and from these results, one might perceive a doublet but the resolution is insufficient to make a clear judgement. This is all the information that I have at the moment. Have you tried these two antibodies with different types of samples?
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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Predicted band size : 108 kDa
Samples: Whole cell lysate (30 µg) from HeLa cells that were either untreated or treated with alkaline phosphatase (AP). Antibodies: ab1187 used at 0.1 µg/ml. After probing with the phospho-specific antibodies, ab4461 was used at 0.2 µg/ml to show total MCM2. Detection: Chemiluminescence with a 1 minute exposure.
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