Loading...
You have changed your country from
to
. Please be aware that this will change the currency in the purchasing process.
Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Overview
Product name
Anti-MCM2 antibody
MCM2 抗体 (25件) 一覧
Description
Rabbit polyclonal to MCM2
Tested applications
ICC/IF, WB, IHC-Pmore details
Cross reactivity
Reacts with
Mouse, Human
Predicted to work with
Rat
Immunogen
Synthetic peptide corresponding to N terminal of human MCM2 protein.
Positive control
HeLa cells. Breast carcinoma.
Properties
Form
Liquid
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage buffer
Preservative: 0.1% Sodium Azide
Constituents: 1% BSA, 10mM PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Protein A purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Applications
Show applications key
Our Abpromise guarantee covers the use of ab31159 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use at an assay dependent dilution.
WB: Use at an assay dependent dilution. Predicted molecular weight: 102 kDa.
IHC-P: 1/100Perform heat mediated antigen retrieval via the pressure cooker method before commencing with IHC staining protocol.
Target
Function
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
Sequence similarities
Belongs to the MCM family.
Contains 1 MCM domain.
Post-translational
modifications
Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP49736
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- BM28 antibody
- CCNL 1 antibody
- CCNL1 antibody
see all
Database links
- Entrez Gene: 4171 Human
- Entrez Gene: 17216 Mouse
- Entrez Gene: 312538 Rat
- Omim: 116945 Human
- SwissProt: P49736 Human
- SwissProt: P97310 Mouse
- Unigene: 477481 Human
- Unigene: 16711 Mouse
see all
Anti-MCM2 antibody images:
Immunohistochemistry (Formalin-fixed paraffin-embedded sections) - MCM2 antibody (ab31159)
Immunohistochemical analysis of MCM2 expression in formalin fixed paraffin embedded human breast carcinoma tissue section, using 1/100 ab31159.
Western blot - MCM2 antibody (ab31159)
Predicted band size : 102 kDa
Soluble or chromatin-bound protein prepared from MEFs was electrophoresed on PAGE gels, electrotransferred, and the blots were immunolabeled with ab31159. The bands correspond to the predicted molecular weights of these proteins, and for MCM2, the identity of the band was verifed by RNAi knockdown and transient overexpression in NIH 3T3 cells.
Image from Chuang CH et al, PLoS Genet. 2010 Sep 9;6(9). pii: e1001110, Fig 1.
Immunocytochemistry/ Immunofluorescence - MCM2 antibody (ab31159)
ab31159 staining MCM2in Human HeLa cells by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with formaldehyde, permeabilized with Triton 0.1% in PBS and blocked with 3% BSA for 1 hour at 24°C. Samples were incubated with primary antibody (1/100 in PBS + 3% BSA) for 1 hour at 37°C. An Alexa Fluor®555-conjugated Goat anti-rabbit IgG polyclonal (1/500) was used as the secondary antibody. Nuclei were counterstained in blue.
This image is courtesy of an Abreview submitted by Nina Marinsek
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-MCM2 antibody (ab31159)
ab31159 staining MCM2 in human tonsil tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).Tissue was fixed in formaldehyde and a heat mediated antigen retrieval step was performed using EDTA pH9.0. Samples were then incubated with ab31159 at a 1/300 for 20 minutes at 25°C. The secondary used was an undiluted HRP conjugated goat anti-mouse-rabbit polyclonal.
This image is courtesy of an anonymous Abreview.
References for Anti-MCM2 antibody (ab31159)
This product has been referenced in:
- Kueh AJet al. HBO1 Is Required for H3K14 Acetylation and Normal Transcriptional Activity during Embryonic Development. Mol Cell Biol 31:845-860 (2011). IHC-P; Mouse.Read more (PubMed: 21149574) »
- Hartford SAet al. Minichromosome maintenance helicase paralog MCM9 is dispensible for DNA replication but functions in germ-line stem cells and tumor suppression. Proc Natl Acad Sci U S A 108:17702-7 (2011). WB; Mouse.Read more (PubMed: 21987787) »
See all 6 publications for this product
Publishing research using ab31159? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"