Application
Western blot
Sample
Human Cell lysate - nuclear (SupT1, Jurkat, Hela cells)
Loading amount
15 µg
Specification
SupT1, Jurkat, Hela cells
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5%
Other product details
Dilution
1/1000
Incubation time
12 hour(s) and 0 minute(s)
Secondary antibody
Name
Non-Abcam antibody was used: goat anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 680
Detection
Detection method
Odyssey, IR imaging
Exposure
5 second(s)
Bands
Specific: 120 kDa
Positive control
All are dividing cells, express MCM2
Additional data
Additional Notes
Bands from left to right are SupT1, Jurkat, and Hela cell lysates
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Nov 08 2006