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Read our guarantee »Products:Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Anti-MCM2 antibody [CRCT2.1 (D1.9H5) ]
MCM2 抗体 (25件) 一覧
Mouse monoclonal [CRCT2.1 (D1.9H5) ] to MCM2
Recognises human Mcm2
IHC-P, IHC-Fr, WBmore details
Reacts with
Human
Bacterially expressed human Mcm2
Tonsil, any tissue with high proliferative index
Liquid
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
PBS with 0.1% sodium azide
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Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant
Monoclonal
CRCT2.1 (D1.9H5)
Sp2
IgG1
Epigenetics and Nuclear Signaling >> DNA / RNA >> DNA Synthesis >> Other
Western blot - MCM2 antibody [CRCT2.1 (D1.9H5) ] (ab6153)
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Our Abpromise guarantee covers the use of ab6153 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P: 1/200Perform heat mediated antigen retrieval before commencing with IHC staining protocol.
IHC-Fr: 1/500 - 1/1000.
WB: Use at an assay dependent dilution. Detects a band of approximately 110 kDa (predicted molecular weight: 100 kDa).
Acts as component of the MCM2-7 complex (MCM complex) which is the putative replicative helicase essential for 'once per cell cycle' DNA replication initiation and elongation in eukaryotic cells. The active ATPase sites in the MCM2-7 ring are formed through the interaction surfaces of two neighboring subunits such that a critical structure of a conserved arginine finger motif is provided in trans relative to the ATP-binding site of the Walker A box of the adjacent subunit. The six ATPase active sites, however, are likely to contribute differentially to the complex helicase activity. Required for the entry in S phase and for cell division.
Belongs to the MCM family.
Contains 1 MCM domain.
Phosphorylated on Ser-108 by ATR in proliferating cells. Ser-108 proliferation is increased by genotoxic agents. Ser-40 is mediated by the CDC7-DBF4 and CDC7-DBF4B complexes, while Ser-53 phosphorylation is only mediated by the CDC7-DBF4 complex. Phosphorylation by the CDC7-DBF4 complex during G1/S phase is required for the initiation of DNA replication.
Nucleus.
Target information above from: UniProt accessionP49736
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Western blot - MCM2 antibody [CRCT2.1 (D1.9H5) ] (ab6153)
![Western blot - MCM2 antibody [CRCT2.1 (D1.9H5) ] (ab6153)](/ps/datasheet/Images/6/ab6153/ab6153.jpg)
Predicted band size : 100 kDa
Western blot using HeLa OHIO extract. The antibody recognizes a doublet band and these migrate closely together. Review by Minna Makiniemi submitted 16 April 2004.
This product has been referenced in:
See all 6 publications for this product
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