Anti-MCM2 antibody [CRCT2.1 (D1.9H5) ] (ab6153)

A customer needs an ab to human MCM2 for IHC and IF. The closest I found is ab6153, but it was not tested for IF. Would you expect it to be applicable for IF? (We know that the abpromise will not be valid for IF).

ANSWER:

The antibody has good chances of working in other types of immunohistochemistry other than those already tested as it has the ability to recognise the native form of the protein. If the customer means IF as in ICC/IF I would recommend to try fixing the cells in PFA 5-10min. If the customer means IF as in IHC-P or IHC-Fr with an IF detection method I would recommend to follow our recommendation for those methods but use an IF amplification kit (i.e either a biotin secondary + extra-avidin/FITC or TRITC method or more sensitive methods which come from Kits eg the TSA kit).

I hope this will help your customer,

I am going to buy your products, MCM2 antibody. But I found several same antibody from your company. This antibody will be used in Western blot. Could you tell me what difference between them(such as, ab1896, ab6153,ab4461)? Are they all antibody for phosphoMCM2? Could you help me to decide which I had better to choose?

Many thanks

ANSWER:

All the information available for this product is listed on the on-line datasheet (price, datasheet, immunogen, publication, suitability, cross-reactivity). I suggest you take a look at the datasheets for these particular products in order to ascertain their level of usefulness in your experimental procedures. I'm sorry for not being able to give you more guidance. If you require additional information to that provided on the product datasheet, please contact us again and we will gladly assist you.

I need to know it's success rate for immunhistochemistry on formalin fixed, paraffin embedded tissues.

ANSWER:

All the information we have for this product for use in IHC is on the datasheet, including recommended dilutions and advice for antigen retrieval. I also suggest that you click through to the review submitted by a researcher who successfully used this antibody in IHC on human lung cancer paraffin embedded tissue. In terms of the "success rate" this will depend on a number variables, including the species that you are studying. Species cross-reactivity for this product has not been tested. However, as discussed this antibody works very well in IHC on human tissue.