Application
Immunohistochemistry (Frozen sections)
Sample
Mouse Tissue sections (brains 25um sections)
Permeabilization
Yes - 1xPBT
Specification
brains 25um sections
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 1% · Temperature: 17°C
Fixative
formalin
Other product details
Incubation time
36 hour(s) and 0 minute(s) · Temperature: 5°C · Diluent: 1% BSA/PBS
Concentration
2.5 µg/ml
Secondary antibody
Dilution
1/1000
Name
Non-Abcam antibody was used: Jackson donkey anti-rabbit 711-065-152
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Host species: Rabbit
Clonality: Polyclonal
Conjugation: Biotin
Additional data
Additional Notes
Sections containing dense MC4R expression (e.g. PVH, Liu et al. 2003) and sections containing no MC4R expression (e.g. thalamus) were examined at a range of concentrations (1:50 and 1:150). A positive control was not used, but this technique has been used effectively by our lab for the past 8 years. MC4R was not detected using this protocol.
Liu et al. 2003
http://www.jneurosci.org/content/23/18/7143.full.pdf
Abcam response
Many thanks for your feedback on ab106596; your data suggests that this antibody is not suitable for us in IHC-Fr in mouse brain
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Sep 04 2012