Application
Immunoprecipitation
Immuno-precipitation step
Protein G
Sample
Mouse Cell lysate - nuclear (ES cells)
Specification
ES cells
Total protein in input
1000 µg
Other product details
Incubation time
24 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: IP buffer
Additional data
Additional Notes
This antibody was tested only in mouse cells.
Samples 1, 3 and 5: Mbd3 +/+,
Samples 2, 4 and 6: Mbd3-/-,
Samples 1and 2: Input,
Samples 3 and 4: IP with ab91458,
Samples 5 and 6: IP with ab157464.
Abcam response
Thank you for sharing this data with us. ab91458 has not previously been tested on mouse lysates. However, due to a lack of sequence homology in the immunogen region, we do not expect this antibody to work on this species. We hope that this information is useful to other customers.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
Abcam user community
Verified customer
投稿 Nov 01 2013