Anti-MBD2 + MBD3 抗体 [106B691] (ab45027)
Key features and details
- Mouse monoclonal [106B691] to MBD2 + MBD3
- Suitable for: IHC-P, Flow Cyt (Intra), ICC/IF, WB
- Reacts with: Human
- Isotype: IgG1
製品の概要
-
製品名
Anti-MBD2 + MBD3 antibody [106B691] -
製品の詳細
Mouse monoclonal [106B691] to MBD2 + MBD3 -
由来種
Mouse -
アプリケーション
適用あり: IHC-P, Flow Cyt (Intra), ICC/IF, WBmore details -
種交差性
交差種: Human
交差が予測される動物種: Mouse, Rat, Chicken, Xenopus laevis, Zebrafish -
免疫原
Synthetic peptide corresponding to Human MBD2 + MBD3 aa 200-300. Synthetic peptide:
CKAFMVTDEDIRKQEE
, corresponding to amino acids 215-230 of Human MBD3
Database link: Q9UBB5 -
ポジティブ・コントロール
- HeLa cells (nuclear fraction). IHC-P: Human placenta tissue Flow Cyt (Intra): HeLa cells.
-
特記事項
The theoretical molecular weight of human MBD2 (411 amino acids) is 45 kD and human MBD3 (291 amino acids) is 33 kD.
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
-
製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
バッファー
pH: 7.4
Preservative: 0.02% Sodium azide
Constituent: PBS -
Concentration information loading...
-
精製度
Protein G purified -
一次抗体 備考
The theoretical molecular weight of human MBD2 (411 amino acids) is 45 kD and human MBD3 (291 amino acids) is 33 kD. -
ポリ/モノ
モノクローナル -
クローン名
106B691 -
アイソタイプ
IgG1 -
軽鎖の種類
kappa -
研究分野
関連製品
-
ChIP Related Products
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab45027の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
---|---|---|
IHC-P |
1/300.
|
|
Flow Cyt (Intra) |
Use 1µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
|
ICC/IF |
Use at an assay dependent concentration.
|
|
WB | (1) |
Use a concentration of 2 µg/ml. Predicted molecular weight: 33, 45 kDa.
|
特記事項 |
---|
IHC-P
1/300. |
Flow Cyt (Intra)
Use 1µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody. |
ICC/IF
Use at an assay dependent concentration. |
WB
Use a concentration of 2 µg/ml. Predicted molecular weight: 33, 45 kDa. |
ターゲット情報
-
関連性
DNA methylation, or the addition of methyl groups to cytosine bases in the dinucleotide CpG, is imperative to proper development and regulates gene expression. The methylation pattern involves the enzymatic processes of methylation and demethylation. The demethylation enzyme was recently found to be a mammalian protein, which exhibits demethylase activity associated to a methyl-CpG-binding domain (MBD). The enzyme is able to revert methylated cytosine bases to cytosines within the particular dinucleotide sequence mdCpdG by catalyzing the cleaving of the methyl group as methanol. MeCP2 and MBD1 (PCM1) are first found to repress transcription by binding specifically to methylated DNA. MBD2 and MBD4 (also known as MED1) were later found to colocalize with foci of heavily methylated satellite DNA and believed to mediate the biological functions of the methylation signal. Surprisingly, MBD3 does not bind methylated DNA both in vivo and in vitro. MBD1, MBD2, MBD3, and MBD4 are found to be expressed in somatic tissues, but the expression of MBD1 and MBD2 is reduced or absent in embryonic stem cells, which are known to be deficient in MeCP1 activity. MBD4 have homology to bacterial base excision repair DNA N-glycosylases/lyases. In some microsatellite unstable tumors MBD4 is mutated at an exonic polynucleotide tract. -
細胞内局在
Nuclear -
参照データベース
- Entrez Gene: 497269 Chicken
- Entrez Gene: 53615 Human
- Entrez Gene: 8932 Human
- Entrez Gene: 17191 Mouse
- Entrez Gene: 17192 Mouse
- Entrez Gene: 362834 Rat
- Entrez Gene: 680172 Rat
- Entrez Gene: 398135 Xenopus laevis
see all -
別名
- Demethylase antibody
- DMTase antibody
- MBD 2 antibody
see all
画像
-
All lanes : Anti-MBD2 + MBD3 antibody [106B691] (ab45027) at 2 µg/ml
Lane 1 : HeLa cell lysate (nuclear fraction) without immunising peptide
Lane 2 : HeLa cell lysate (nuclear fraction) with immunising peptide
Predicted band size: 33, 45 kDa
Observed band size: 33,45 kDa why is the actual band size different from the predicted?
Additional bands at: 160 kDa, 40 kDa. We are unsure as to the identity of these extra bands. -
Overlay histogram showing HeLa cells stained with ab45027 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab45027, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2µg/1x106 cells ) used under the same conditions. Acquisition of >5,000 events was performed.
-
ab45027 at 2µg/ml staining MBD2 + MBD3 in human glioma tissue by Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections).
Tissue was fixed in paraformaldehyde and a heat mediated antigen retrieval step was performed and samples were blocked using 10% BSA. The secondary used was an HRP conjugated anti rabbit/mouse mix. -
Immunohistochemistry analysis of immersion fixed paraffin-embedded sections of human placenta labeling MBD2 + MBD3 with ab45027 at 1/300 for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody. Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue).
プロトコール
データシートおよび資料
-
SDS download
-
Datasheet download
参考文献 (10)
ab45027 は 10 報の論文で使用されています。
- Kraushaar DC et al. The gene repressor complex NuRD interacts with the histone variant H3.3 at promoters of active genes. Genome Res 28:1646-1655 (2018). PubMed: 30254051
- Xu M et al. MeCP2 suppresses LIN28A expression via binding to its methylated-CpG islands in pancreatic cancer cells. Oncotarget 7:14476-85 (2016). WB . PubMed: 26910839
- Gómez-Del Arco P et al. The Chromatin Remodeling Complex Chd4/NuRD Controls Striated Muscle Identity and Metabolic Homeostasis. Cell Metab 23:881-92 (2016). PubMed: 27166947
- Teperek M et al. Sperm and spermatids contain different proteins and bind distinct egg factors. Int J Mol Sci 15:16719-40 (2014). WB ; Frog . PubMed: 25244019
- Kim JY et al. Specificity in circadian clock feedback from targeted reconstitution of the NuRD corepressor. Mol Cell 56:738-48 (2014). PubMed: 25453762
- Zhu D et al. Overexpression of MBD2 in glioblastoma maintains epigenetic silencing and inhibits the antiangiogenic function of the tumor suppressor gene BAI1. Cancer Res 71:5859-70 (2011). PubMed: 21724586
- Mastroeni D et al. Epigenetic changes in Alzheimer's disease: decrements in DNA methylation. Neurobiol Aging 31:2025-37 (2010). IHC-Fr . PubMed: 19117641
- Mastroeni D et al. Epigenetic differences in cortical neurons from a pair of monozygotic twins discordant for Alzheimer's disease. PLoS One 4:e6617 (2009). IHC-FoFr ; Human . PubMed: 19672297
- Thatcher KN et al. Homologous pairing of 15q11-13 imprinted domains in brain is developmentally regulated but deficient in Rett and autism samples. Hum Mol Genet 14:785-97 (2005). PubMed: 15689352
- Balmer D et al. MECP2 mutations in Rett syndrome adversely affect lymphocyte growth, but do not affect imprinted gene expression in blood or brain. Hum Genet 110:545-52 (2002). PubMed: 12107440