Application
Western blot
Sample
Human Tissue lysate - whole (skin keratinocytes)
Gel Running Conditions
Reduced Denaturing (AnyKd from BioRad)
Loading amount
40 µg
Specification
skin keratinocytes
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 22°C · Diluent: TBS-T 0.2%
Dilution
1/1000
Secondary antibody
Dilution
1/10000
Name
Non-Abcam antibody was used: anti-mouse HRP-linked
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Horse
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Detection
Detection method
ECL
Exposure
1 minute(s) and 0 second(s)
Bands
Specific: 34 kDa Non-specific: 43 kDa
Positive control
my sample
Additional data
Additional Notes
0.2 um nitrocellulose membrane
fast transfer with TransBlot Turbo
Lane 1 : whole cell lysate from skin keratinocytes, 40 ug
Lane 2 : Molecular weight markers
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
MR. Julien Coutier
Verified customer
投稿 Feb 07 2017