Anti-MAX 抗体 [73C5a] (ab53570)
Key features and details
- Mouse monoclonal [73C5a] to MAX
- Suitable for: ChIP, Flow Cyt, Dot blot, WB
- Reacts with: Human, Recombinant fragment
- Isotype: IgG2b
製品の概要
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製品名
Anti-MAX antibody [73C5a]
MAX 一次抗体 製品一覧 -
製品の詳細
Mouse monoclonal [73C5a] to MAX -
由来種
Mouse -
アプリケーション
適用あり: ChIP, Flow Cyt, Dot blot, WBmore details -
種交差性
交差種: Human, Recombinant fragment -
免疫原
Recombinant human MAX
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
バッファー
pH: 7.40
Preservative: 0.05% Sodium azide
Constituents: 1% BSA, 0.03% Potassium phosphate, 0.812% Sodium chloride, 0.1312% Sodium phosphate, 0.0225% Potassium chloride, PBS -
Concentration information loading...
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精製度
Protein G purified -
特記事項(精製)
ab53570 was purified using protein G column chromatography from culture supernatant of hybridoma cultured in a medium containing bovine IgG-depleted (approximately 95%) fetal bovine serum and filtered through a 0.22µm membrane. -
ポリ/モノ
モノクローナル -
クローン名
73C5a -
アイソタイプ
IgG2b -
研究分野
関連製品
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ChIP Related Products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab53570の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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ChIP |
Use at an assay dependent concentration. PubMed: 20797623
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Flow Cyt |
Use 1µg for 106 cells.
ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
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Dot blot |
Use at an assay dependent concentration.
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WB | (1) |
Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 18 kDa).
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特記事項 |
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ChIP
Use at an assay dependent concentration. PubMed: 20797623 |
Flow Cyt
Use 1µg for 106 cells. ab170192 - Mouse monoclonal IgG2b, is suitable for use as an isotype control with this antibody. |
Dot blot
Use at an assay dependent concentration. |
WB
Use at an assay dependent concentration. Detects a band of approximately 34 kDa (predicted molecular weight: 18 kDa). |
ターゲット情報
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機能
Transcription regulator. Forms a sequence-specific DNA-binding protein complex with MYC or MAD which recognizes the core sequence 5'-CAC[GA]TG-3'. The MYC:MAX complex is a transcriptional activator, whereas the MAD:MAX complex is a repressor. May repress transcription via the recruitment of a chromatin remodeling complex containing H3 'Lys-9' histone methyltransferase activity. -
組織特異性
High levels found in the brain, heart and lung while lower levels are seen in the liver, kidney and skeletal muscle. -
関連疾患
Pheochromocytoma -
配列類似性
Belongs to the MAX family.
Contains 1 bHLH (basic helix-loop-helix) domain. -
翻訳後修飾
Reversible lysine acetylation might regulate the nuclear-cytoplasmic shuttling of specific Max complexes. -
細胞内局在
Nucleus. Cell projection, dendrite. - Information by UniProt
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参照データベース
- Entrez Gene: 4149 Human
- Omim: 154950 Human
- SwissProt: P61244 Human
- Unigene: 285354 Human
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別名
- bHLHd4 antibody
- bHLHd5 antibody
- bHLHd6 antibody
see all
画像
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Anti-MAX antibody [73C5a] (ab53570) + immunised recombinant protein
Predicted band size: 18 kDa
Observed band size: 34 kDa why is the actual band size different from the predicted? -
Overlay histogram showing A549 cells stained with ab53570 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab53570, 1µg/1x106 cells) for 30 min at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22ºC. Isotype control antibody (black line) was mouse IgG2b [PLPV219] (ab91366, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. This antibody gave a positive signal in A549 cells fixed with 4% paraformaldehyde (10 min)/permeabilized with 0.1% PBS-Tween for 20 min used under the same conditions.
データシートおよび資料
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Datasheet download
参考文献 (11)
ab53570 は 11 報の論文で使用されています。
- Lu W et al. The CARM1-p300-c-Myc-Max (CPCM) transcriptional complex regulates the expression of CUL4A/4B and affects the stability of CRL4 E3 ligases in colorectal cancer. Int J Biol Sci 16:1071-1085 (2020). PubMed: 32140074
- Khazeem MM et al. Transcription of carbonyl reductase 1 is regulated by DNA topoisomerase II beta. FEBS Lett 594:3395-3405 (2020). PubMed: 32767399
- Mudersbach T et al. Epigenetic control of the angiotensin-converting enzyme in endothelial cells during inflammation. PLoS One 14:e0216218 (2019). PubMed: 31042763
- Lu YC et al. The Molecular Signature of Megakaryocyte-Erythroid Progenitors Reveals a Role for the Cell Cycle in Fate Specification. Cell Rep 25:2083-2093.e4 (2018). PubMed: 30463007
- Studd JB et al. Genetic predisposition to B-cell acute lymphoblastic leukemia at 14q11.2 is mediated by a CEBPE promoter polymorphism. Leukemia N/A:N/A (2018). PubMed: 29977016