製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MAT2B
  • アプリケーション
    適用あり: ICC/IF, WBmore details
  • 種交差性
    交差種: Human
    交差が予測される動物種: Mouse, Rat, Rabbit, Horse, Chicken, Guinea pig, Cow, Cat, Dog, Pig
  • 免疫原

    Synthetic peptide corresponding to a region within N terminal amino acids 36-85 (EFQQNNWHAV GCGFRRARPK FEQVNLLDSN AVHHIIHDFQ PHVIVHCAAE) of human MAT2B (NP_877725).

  • ポジティブ・コントロール
    • Human fetal liver lysate

製品の特性

アプリケーション

Our Abpromise guarantee covers the use of ab86506 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
ICC/IF Use a concentration of 5 µg/ml.
WB Use a concentration of 1 µg/ml. Predicted molecular weight: 36 kDa. Good results were obtained when blocked with 5% non-fat dry milk in 0.05% PBS-T.

ターゲット情報

  • 機能
    Non-catalytic regulatory subunit of S-adenosylmethionine synthetase 2 (MAT2A), an enzyme that catalyzes the formation of S-adenosylmethionine from methionine and ATP. Regulates the activity of S-adenosylmethionine synthetase 2 by changing its kinetic properties, rendering the enzyme more susceptible to S-adenosylmethionine inhibition.
  • 組織特異性
    Widely expressed.
  • パスウェイ
    Amino-acid biosynthesis; S-adenosyl-L-methionine biosynthesis; S-adenosyl-L-methionine from L-methionine: step 1/1.
  • 配列類似性
    Belongs to the dTDP-4-dehydrorhamnose reductase family. MAT2B subfamily.
  • Information by UniProt
  • 参照データベース
  • 別名
    • 1110064C04Rik antibody
    • 2410018D16Rik antibody
    • AI182287 antibody
    • AU022853 antibody
    • Beta regulatory subunit of methionine adenosyltransferase antibody
    • dTDP 4 keto 6 deoxy D glucose 4 reductase antibody
    • DTDP-4-keto-6-deoxy-D-glucose 4-reductase antibody
    • MAT II antibody
    • MAT II beta antibody
    • Mat2b antibody
    • MAT2B_HUMAN antibody
    • MATIIbeta antibody
    • Methionine adenosyltransferase 2 beta subunit antibody
    • Methionine adenosyltransferase 2 subunit beta antibody
    • Methionine adenosyltransferase II beta antibody
    • MGC12237 antibody
    • MSTP045 antibody
    • Nbla02999 antibody
    • OTTMUSP00000005600 antibody
    • Putative dTDP-4-keto-6-deoxy-D-glucose 4-reductase antibody
    • Putative protein product of Nbla02999 antibody
    • RP23-382C18.2 antibody
    • SDR23E1 antibody
    • Short chain dehydrogenase/reductase family 23E member 1 antibody
    • TGR antibody
    see all

画像

  • Anti-MAT2B antibody (ab86506) at 1 µg/ml + human fetal liver lysate at 10 µg

    Secondary
    anti-Rabbit IgG HRP at 1/50000 dilution

    Predicted band size : 36 kDa
    Observed band size : 36 kDa
  • ICC/IF image of ab86506 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab86506, 5µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

参考文献

This product has been referenced in:
  • Borrego SL  et al. Metabolic changes associated with methionine stress sensitivity in MDA-MB-468 breast cancer cells. Cancer Metab 4:9 (2016). WB . Read more (PubMed: 27141305) »

See 1 Publication for this product

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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