Anti-GWL 抗体 (ab86387)
Key features and details
- Rabbit polyclonal to GWL
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-GWL antibody
GWL 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to GWL -
由来種
Rabbit -
アプリケーション
適用あり: WB, IP, IHC-Pmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human GWL aa 550-600.
Database link: NP_116233.2 -
ポジティブ・コントロール
- Hela and 293T whole cell lysates
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
バッファー
pH: 7
Preservative: 0.09% Sodium azide
Constituent: Tris citrate/phosphate -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Positive Controls
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab86387の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 97 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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特記事項 |
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WB
1/2000 - 1/10000. Predicted molecular weight: 97 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/500 - 1/2000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ターゲット情報
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機能
Serine/threonine kinase that plays a key role in M phase by acting as a regulator of mitosis entry and maintenance. Acts by promoting the inactivation of protein phosphatase 2A (PP2A) during M phase: does not directly inhibit PP2A but acts by mediating phosphorylation and subsequent activation of ARPP19 and ENSA at 'Ser-62' and 'Ser-67', respectively. ARPP19 and ENSA are phosphatase inhibitors that specifically inhibit the PPP2R2D (PR55-delta) subunit of PP2A. Inactivation of PP2A during M phase is essential to keep cyclin-B1-CDK1 activity high. Following DNA damage, it is also involved in checkpoint recovery by being inhibited. Phosphorylates histone protein in vitro; however such activity is unsure in vivo. May be involved in megakaryocyte differentiation. -
関連疾患
Defects in MASTL are the cause of thrombocytopenia type 2 (THC2) [MIM:188000]. Thrombocytopenia is defined by a decrease in the number of platelets in circulating blood, resulting in the potential for increased bleeding and decreased ability for clotting. -
配列類似性
Belongs to the protein kinase superfamily. AGC Ser/Thr protein kinase family.
Contains 1 AGC-kinase C-terminal domain.
Contains 1 protein kinase domain. -
翻訳後修飾
Phosphorylation at Thr-741 by CDK1 during M phase activates its kinase activity (By similarity). Maximum phosphorylation occurs in prometaphase. -
細胞内局在
Cytoplasm > cytoskeleton > centrosome. Nucleus. Cleavage furrow. During interphase is mainly nuclear, upon nuclear envelope breakdown localizes at the cytoplasm and during mitosis at the centrosomes. Upon mitotic exit moves to the cleavage furrow. - Information by UniProt
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参照データベース
- Entrez Gene: 84930 Human
- Omim: 608221 Human
- SwissProt: Q96GX5 Human
- Unigene: 276905 Human
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別名
- 2700091H24Rik antibody
- C88295 antibody
- FLJ14813 antibody
see all
画像
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All lanes : Anti-GWL antibody (ab86387) at 0.1 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Developed using the ECL technique.
Predicted band size: 97 kDa
Observed band size: 118 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma (left) and lung carcinoma (right) tissues labelling GWL with ab86387 at 1/1000 (1µg/ml) and 1/500 (2µg/ml). Detection: DAB and a DyLight® 594-conjugated goat anti-rabbit IgG (H+L) (1/100).
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Detection of GWL by Western blot of Immunprecipitate.
ab86387, at 1 µg/ml, staining GWL in HeLa whole cell lysate immunoprecipitated using ab86387 at 3 µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with an exposure time of 10 seconds.
プロトコール
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (8)
ab86387 は 8 報の論文で使用されています。
- Närvä E et al. MASTL is enriched in cancerous and pluripotent stem cells and influences OCT1/OCT4 levels. iScience 25:104459 (2022). PubMed: 35677646
- Taskinen ME et al. MASTL promotes cell contractility and motility through kinase-independent signaling. J Cell Biol 219:N/A (2020). PubMed: 32311005
- Cetti E et al. Mitosis perturbation by MASTL depletion impairs the viability of thyroid tumor cells. Cancer Lett 442:362-372 (2019). PubMed: 30445205
- Cao L et al. Inflammatory cytokine-induced expression of MASTL is involved in hepatocarcinogenesis by regulating cell cycle progression. Oncol Lett 17:3163-3172 (2019). PubMed: 30867746
- Serpico AF et al. Wee1 Rather Than Plk1 Is Inhibited by AZD1775 at Therapeutically Relevant Concentrations. Cancers (Basel) 11:N/A (2019). PubMed: 31200459
- Anania M et al. Identification of thyroid tumor cell vulnerabilities through a siRNA-based functional screening. Oncotarget 6:34629-48 (2015). WB . PubMed: 26431489
- Chen YL et al. ENSA expression correlates with attenuated tumor propagation in liver cancer. Biochem Biophys Res Commun 442:56-61 (2013). IP ; Human . PubMed: 24211627
- Potapova TA et al. Mitotic progression becomes irreversible in prometaphase and collapses when Wee1 and Cdc25 are inhibited. Mol Biol Cell 22:1191-206 (2011). WB ; Human . PubMed: 21325631