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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Overview
Product name
Anti-MASH1/Achaete-scute homolog 1 antibody
MASH1/Achaete-scute homolog 1 抗体 (7件) 一覧
Description
Rabbit polyclonal to MASH1/Achaete-scute homolog 1
Tested applications
WB, ICC/IFmore details
Cross reactivity
Reacts with
Mouse, Rat, Human, Zebrafish
Predicted to work with
Chicken, Dog, Xenopus laevis, Rhesus monkey
Immunogen
Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human MASH1/Achaete-scute homolog 1.
(Peptide available as ab74622.)
Positive control
This antibody gave a positive signal in the following lysates: DU 145 Whole Cell Rat Brain Tissue Mouse Brain Tissue
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4
Concentration
Concentration information loading...
Purity
Immunogen affinity purified
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Neurogenesis
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Applications
Show applications key
Our Abpromise guarantee covers the use of ab74065 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
WB: Use a concentration of 1 µg/mlDetects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
ICC/IF: Use a concentration of 5 µg/ml
Target
Function
May play a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Activates transcription by binding to the E box (5'-CANNTG-3').
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP50553
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Achaete scute complex homolog 1 antibody
- Achaete scute complex homolog like 1 antibody
- Achaete scute complex homologue 1 antibody
see all
Database links
- Entrez Gene: 386573 Chicken
- Entrez Gene: 429 Human
- Entrez Gene: 17172 Mouse
- Entrez Gene: 64186 Rat
- Entrez Gene: 378517 Xenopus laevis
- Omim: 100790 Human
- SwissProt: P50553 Human
- SwissProt: Q02067 Mouse
see all
Anti-MASH1/Achaete-scute homolog 1 antibody images:
Western blot - MASH1/Achaete-scute homolog 1 antibody (ab74065)
All lanes : Anti-MASH1/Achaete-scute homolog 1 antibody (ab74065) at 1 µg/ml
Lane 1 : DU 145 (Human prostate carcinoma cell line) Whole Cell Lysate
Lane 2 : Brain (Rat) Tissue Lysate
Lane 3 : Brain (Mouse) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Secondary
Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 25 kDa
Observed band size : 25 kDa
Exposure time : 3 minutes
Immunocytochemistry/ Immunofluorescence - Anti-MASH1/Achaete-scute homolog 1 antibody (ab74065)
ICC/IF image of ab74065 stained Hek293 cells. The cells were 100% methanol fixed (5 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab74065, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit Igg (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM. This antibody also gave a positive result in 100% methanol fixed (5 min) HeLa and HepG2 cells at 5µg/ml.
Western blot - Anti-MASH1/Achaete-scute homolog 1 antibody (ab74065)
All lanes : Anti-MASH1/Achaete-scute homolog 1 antibody (ab74065) at 1 µg/ml
Lane 1 : Marker
Lane 2 : Zebrafish brain homogenate at 20 µg
Lane 3 : Mouse brain homogenate at 20 µg
Secondary
Goat polyclonal to Rabbit IgG – H&L – Pre-Adsorbed (HRP) at 1/6000 dilution
developed using the ECL technique
Performed under reducing conditions.
Predicted band size : 25 kDa
Observed band size : 26 kDa (why is the actual band size different from the predicted?)
Exposure time : 5 minutes
References for Anti-MASH1/Achaete-scute homolog 1 antibody (ab74065)
ab74065 has not yet been referenced specifically in any publications.
Publishing research using ab74065? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"