MASH1/Achaete-scute homolog 1 antibody (ab74065)

Product Name 

MASH1/Achaete-scute homolog 1 antibody

Product type 

Primary antibodies

Description 

Rabbit polyclonal to MASH1/Achaete-scute homolog 1

Immunogen 

Synthetic peptide conjugated to KLH derived from within residues 200 to the C-terminus of Human MASH1/Achaete-scute homolog 1.

(Peptide available as ab74622.)

Reacts with 

Hu, Ms, Rat

Predicted to react 
(species key)

Chk, Dog, RMk, Xl, Zfsh
(Due to sequence homology)

Tested applications 
(see key)

ICC/IF, WB

Abreviews 

Customer reviews (see key)

Read about Abreviews (Read all Abreviews)

Application notes 
(see key)

Recommended dilutions
ICC/IF: Use at a concentration of 5 µg/ml.
WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).


Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.

Positive control 
(see definition)

This antibody gave a positive signal in the following lysates:
DU 145 Whole Cell
Rat Brain Tissue
Mouse Brain Tissue

Cellular localization 

Nuclear

Research areas 

Neuroscience >> Neurology process >> Neurogenesis
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH

Relevance 

ACSL1, alternatively titled Hash1 or Mash1, is a member of the basic helix-loop-helix (BHLH) family of transcription factors. Achaete-scute homolog 1 may play a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. It is essential for the generation of olfactory and autonomic neurons and activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. ACSL1 plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. The protein is highly expressed in medullary thyroid cancer and small cell lung cancer and may be a useful marker for these cancers. The presence of a CAG repeat in the gene suggests it may also play a role in tumor formation. Between embryonic days 8.5 and 10.5 it is found in the neuroepithelium of the midbrain and ventral forebrain, as well as in the spinal cord. Between days 10.5 and 12.5 its expression pattern changes from a restricted to a widespread one, it is then found at variable levels in the ventricular zone in all regions of the brain. From day 12.5 to post-natal stages it is also expressed in cells outside of the ventricular zone through the brain, and in addition it is also expressed during development of the olfactory epithelium and neural retina.

Database links 

Raised in 

Rabbit

Clonality 

Polyclonal

Isotype 

IgG

Purity 

Immunogen affinity purified

Storage buffer 

Preservative: 0.02% Sodium Azide
Constituents: 1% BSA, PBS, pH 7.4

Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS

Form 

Liquid

Concentration 

0.300 mg/ml

Storage instructions 

Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.

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