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Western blot - Achaete-scute homolog 1 antibody (ab38557)
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Achaete-scute homolog 1 antibody (ab38557)
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Immunocytochemistry/ Immunofluorescence-MASH1/Achaete-scute homolog 1 antibody(ab38557)
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Product Name
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MASH1/Achaete-scute homolog 1 antibody
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MASH1/Achaete-scute homolog 1 抗体 (7件) 一覧
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Product type
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Primary antibodies
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Description
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Rabbit polyclonal to MASH1/Achaete-scute homolog 1
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Immunogen
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Synthetic peptide (10-30 aa in length) at the N-term of first 100 aa of human Achaete-scute homolog 1 conjugated to KLH
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Reacts with
(species key)
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Hu, Ms
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Tested applications
(see key)
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ELISA, ICC/IF, IHC-FoFr, IHC-P, WB
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Abreviews
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Application notes
(see key)
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Recommended dilutions
ELISA: 1/1000.
ICC/IF: Use at a concentration of 1 µg/ml.
IHC-P: Use at an assay dependent dilution.
IHC-FoFr: Use at an assay dependent dilution.
WB: 1/1000 - 1/500. Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
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Positive control
(see definition)
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Human breast carcinoma tissue and mouse lung tissue lysate.
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Cellular localization
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Nuclear
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Research areas
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Neuroscience >> Neurology process >> Neurogenesis
Chromatin and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
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Relevance
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ACSL1, alternatively titled Hash1 or Mash1, is a member of the basic helix-loop-helix (BHLH) family of transcription factors. Achaete-scute homolog 1 may play a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. It is essential for the generation of olfactory and autonomic neurons and activates transcription by binding to the E box (5'-CANNTG-3'). Dimerization with other BHLH proteins is required for efficient DNA binding. ACSL1 plays a role in the neuronal commitment and differentiation and in the generation of olfactory and autonomic neurons. The protein is highly expressed in medullary thyroid cancer and small cell lung cancer and may be a useful marker for these cancers. The presence of a CAG repeat in the gene suggests it may also play a role in tumor formation. Between embryonic days 8.5 and 10.5 it is found in the neuroepithelium of the midbrain and ventral forebrain, as well as in the spinal cord. Between days 10.5 and 12.5 its expression pattern changes from a restricted to a widespread one, it is then found at variable levels in the ventricular zone in all regions of the brain. From day 12.5 to post-natal stages it is also expressed in cells outside of the ventricular zone through the brain, and in addition it is also expressed during development of the olfactory epithelium and neural retina.
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MASH1/Achaete-scute homolog 1 antibody (ab38557)の別名 |
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Database links
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The links below go to external sites and will open in a new browser window |
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Raised in
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Rabbit
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Clonality
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Polyclonal
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Isotype
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IgG
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Purity
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Protein G purified
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Storage buffer
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Preservative: 0.09% Sodium Azide
Constituents: PBS Material safety datasheet (MSDS) for this product:
Sodium Azide MSDS
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Purification notes
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ab38557 was purified through a protein G column and eluted out with both high and low pH buffers and neutralised immediately after elution then followed by dialysis against PBS.
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Form
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Liquid
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Concentration
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0.250 mg/ml
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Storage instructions
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Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
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Abcam では全ての製品情報を一元管理しており、 MASH1/Achaete-scute homolog 1 antibody について私たちが知りうる全ての情報がこのデータシートでご覧いただけます。ご不明な点がありましたら、 お問い合わせください。 |
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See below for MASH1/Achaete-scute homolog 1 antibody images, references, products related to ab38557 and other tools.
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MASH1/Achaete-scute homolog 1 antibody images:
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 Western blot - Achaete-scute homolog 1 antibody (ab38557)
MASH1/Achaete-scute homolog 1 antibody (ab38557) at 1/500 dilution + Mouse lung tissue lysate
Predicted band size : 25 kDa
Observed band size : 25 kDa
Additional bands at : 15 kDa. We are unsure as to the identity of these extra bands.
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Achaete-scute homolog 1 antibody (ab38557)
Formalin fixed and paraffin embedded human breast carcinoma tissue stained with ab38557, followed by AEC staining.
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Immunocytochemistry/ Immunofluorescence-MASH1/Achaete-scute homolog 1 antibody(ab38557)
ICC/IF image of ab38557 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38557, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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References for MASH1/Achaete-scute homolog 1 antibody (ab38557)
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This product has been used in: (two most recent references)
Layman WS et al. Defects in neural stem cell proliferation and olfaction in Chd7 deficient mice indicate a mechanism for hyposmia in human CHARGE syndrome. Hum Mol Genet 18:1909-23 (2009). IHC-FoFr; Mouse. PubMed: 19279158
Cherrington BD et al. NeuroD1 and Mash1 temporally regulate GnRH receptor gene expression in immortalized mouse gonadotrope cells. Mol Cell Endocrinol 295:106-14 (2008). PubMed: 18760324
If you publish research using ab38557 please let us know so that we can cite the reference on this datasheet.
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Search PubMed (MEDLINE) for references to MASH1/Achaete-scute homolog 1
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