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Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Overview
Product name
Anti-MASH1/Achaete-scute homolog 1 antibody
MASH1/Achaete-scute homolog 1 抗体 (7件) 一覧
Description
Rabbit polyclonal to MASH1/Achaete-scute homolog 1
Tested applications
ICC/IF, ELISA, WB, IHC-P, IHC-P, IHC-FoFrmore details
Cross reactivity
Reacts with
Mouse, Human
Immunogen
Synthetic peptide (10-30 aa in length) at the N-term of first 100 aa of human Achaete-scute homolog 1 conjugated to KLH
Positive control
Human breast carcinoma tissue and mouse lung tissue lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C long term. Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: 0.09% Sodium Azide
Constituents: PBS
Concentration
Concentration information loading...
Purity
Protein G purified
Purification notes
ab38557 was purified through a protein G column and eluted out with both high and low pH buffers and neutralised immediately after elution then followed by dialysis against PBS.
Clonality
Polyclonal
Isotype
IgG
Research areas
Neuroscience >> Neurology process >> Neurogenesis
Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
Applications
Show applications key
Our Abpromise guarantee covers the use of ab38557 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
ICC/IF: Use a concentration of 1 µg/ml
ELISA
ELISA: 1/1000ELISA: 1/1000
WB: 1/1000 - 1/500.Detects a band of approximately 25 kDa (predicted molecular weight: 25 kDa).
IHC-P: Use at an assay dependent dilution.
IHC-P: Use at an assay dependent concentration.
IHC-FoFr: Use at an assay dependent dilution. (PubMed: 19279158)
Target
Function
May play a role at early stages of development of specific neural lineages in most regions of the CNS, and of several lineages in the PNS. Essential for the generation of olfactory and autonomic neurons. Activates transcription by binding to the E box (5'-CANNTG-3').
Sequence similarities
Contains 1 basic helix-loop-helix (bHLH) domain.
Cellular localization
Nucleus.
Target information above from: UniProt accessionP50553
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- Achaete scute complex homolog 1 antibody
- Achaete scute complex homolog like 1 antibody
- Achaete scute complex homologue 1 antibody
see all
Database links
- Entrez Gene: 429 Human
- Entrez Gene: 17172 Mouse
- Omim: 100790 Human
- SwissProt: P50553 Human
- SwissProt: Q02067 Mouse
- Unigene: 703025 Human
- Unigene: 712544 Human
- Unigene: 136217 Mouse
Anti-MASH1/Achaete-scute homolog 1 antibody images:
Western blot - Achaete-scute homolog 1 antibody (ab38557)
Anti-MASH1/Achaete-scute homolog 1 antibody (ab38557) at 1/500 dilution + Mouse lung tissue lysate
Predicted band size : 25 kDa
Observed band size : 25 kDa
Additional bands at : 15 kDa. We are unsure as to the identity of these extra bands.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Achaete-scute homolog 1 antibody (ab38557)
Formalin fixed and paraffin embedded human breast carcinoma tissue stained with ab38557, followed by AEC staining.
Immunocytochemistry/ Immunofluorescence-MASH1/Achaete-scute homolog 1 antibody(ab38557)
ICC/IF image of ab38557 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38557, 1µg/ml) overnight at +4°C. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
References for Anti-MASH1/Achaete-scute homolog 1 antibody (ab38557)
This product has been referenced in:
- Hadjivassiliou Get al. The application of cortical layer markers in the evaluation of cortical dysplasias in epilepsy. Acta Neuropathol : (2010). IHC-P; Human.Read more (PubMed: 20411268) »
- Layman WSet al. Defects in neural stem cell proliferation and olfaction in Chd7 deficient mice indicate a mechanism for hyposmia in human CHARGE syndrome. Hum Mol Genet 18:1909-23 (2009). IHC-FoFr; Mouse.Read more (PubMed: 19279158) »
See all 3 publications for this product
Publishing research using ab38557? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"