Products:Epigenetics and Nuclear Signaling >> Transcription >> Domain Families >> HLH / Leucine Zipper >> HLH
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ab38557 has been referenced in 3 publications.
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Anti-MASH1/Achaete-scute homolog 1 antibody (ab38557) at 1/500 dilution + Mouse lung tissue lysate
Predicted band size : 25 kDa
Observed band size : 25 kDa
Additional bands at : 15 kDa. We are unsure as to the identity of these extra bands.
Formalin fixed and paraffin embedded human breast carcinoma tissue stained with ab38557, followed by AEC staining.
ICC/IF image of ab38557 stained HepG2 cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab38557, 1µg/ml) overnight at +4ºC. The secondary antibody (green) was Alexa Fluor® 488 goat anti-rabbit IgG (H+L) used at a 1/1000 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
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