Anti-MAP2 抗体 (ab92434)
Key features and details
- Chicken polyclonal to MAP2
- Suitable for: WB, ICC
- Reacts with: Rat, Human
- Isotype: IgY
製品の概要
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製品名
Anti-MAP2 antibody
MAP2 一次抗体 製品一覧 -
製品の詳細
Chicken polyclonal to MAP2 -
由来種
Chicken -
アプリケーション
適用あり: WB, ICCmore details -
種交差性
交差種: Rat, Human -
免疫原
Recombinant full length protein corresponding to Cow MAP2. Recombinant bovine MAP2 protein expressed in and purified from E. Coli
Database link: F1MEW3 -
特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Liquid -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.065% Sodium azide -
Concentration information loading...
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精製度
IgY fraction -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgY -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab92434の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/20000. Predicted molecular weight: 280 kDa.
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ICC | (1) |
Use at an assay dependent concentration.
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特記事項 |
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WB
1/20000. Predicted molecular weight: 280 kDa. |
ICC
Use at an assay dependent concentration. |
ターゲット情報
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機能
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules. -
配列類似性
Contains 3 Tau/MAP repeats. -
翻訳後修飾
Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). Isoform 2 is probably phosphorylated by PKA at Ser-323, Ser-354 and Ser-386 and by FYN at Tyr-67. -
細胞内局在
Cytoplasm, cytoskeleton. - Information by UniProt
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参照データベース
- Entrez Gene: 4133 Human
- Entrez Gene: 25595 Rat
- Omim: 157130 Human
- SwissProt: P11137 Human
- SwissProt: P15146 Rat
- Unigene: 368281 Human
- Unigene: 10484 Rat
- Unigene: 136301 Rat
see all -
別名
- DKFZp686I2148 antibody
- MAP 2 antibody
- MAP dendrite specific antibody
see all
画像
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ab92434 staining MAP2 in mouse brain neurons by ICC/IF (Immunocytochemistry/immunofluorescence). Cells were fixed with paraformaldehyde and blocked with 5% serum for 1 hour at 20°C. Samples were incubated with primary antibody (1/2500 in PBS) for 24 hours at 4°C. ab150169, a goat anti-chicken Alexa Fluor® 488 (1/500) was used as the secondary antibody.
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Anti-MAP2 antibody (ab92434) at 1/20000 dilution + Rat cerebral cortex lysate
Predicted band size: 280 kDa -
ab92434 at 1/2500 dilution staining MAP2 in mixed neuron/glial cultures by Immunofluorescence. The perikarya and dendrites of neurons are strongly and specifically stained with the MAP2 antibody (red). Cell nuclei are visualized with DAPI DNA stain.
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (45)
ab92434 は 45 報の論文で使用されています。
- Kim YA et al. RNA methyltransferase NSun2 deficiency promotes neurodegeneration through epitranscriptomic regulation of tau phosphorylation. Acta Neuropathol 145:29-48 (2023). PubMed: 36357715
- Ryan SK et al. Microglia ferroptosis is regulated by SEC24B and contributes to neurodegeneration. Nat Neurosci 26:12-26 (2023). PubMed: 36536241
- Mishra S et al. The Alzheimer's gene SORL1 is a regulator of endosomal traffic and recycling in human neurons. Cell Mol Life Sci 79:162 (2022). PubMed: 35226190
- Azevedo C et al. Parkinson's disease and multiple system atrophy patient iPSC-derived oligodendrocytes exhibit alpha-synuclein-induced changes in maturation and immune reactive properties. Proc Natl Acad Sci U S A 119:e2111405119 (2022). PubMed: 35294277
- Teng Z & Gottmann K Hemisynapse Formation Between Target Astrocytes and Cortical Neuron Axons in vitro. Front Mol Neurosci 15:829506 (2022). PubMed: 35386271