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Product name
Anti-MAP2 antibody
MAP2 抗体 (19件) 一覧
Description
Rabbit polyclonal to MAP2
Tested applications
IHC-P, IHC-Fr, WBmore details
Cross reactivity
Reacts with
Mouse, Rat
Immunogen
Synthetic peptide as a part of rat and mouse MAP2, conjugated to an immunogenic carrier protein.
Positive control
Rat brain tissue. Rat DRG. Rat spinal cord tissue. Rat trigeminal nerve. Rat brain lysate.
Properties
Form
Liquid
Storage instructions
Store at +4°C short term (1-2 weeks). Aliquot and store at -20°C (add glycerol to a final volume of 40% for extra stability). Avoid repeated freeze / thaw cycles.
Storage buffer
Preservative: None
Constituents: Whole serum
Purity
Whole antiserum
Clonality
Polyclonal
Isotype
IgG
Research areas
Stem Cells >> Neural Stem Cells >> Neuron Restricted Lineage
Neuroscience >> Cell Type Marker >> Neuron marker >> Dendrite marker
Signal Transduction >> Cytoskeleton / ECM >> Cytoskeleton >> Microtubules >> MT Associated Proteins >> MAP
Neuroscience >> Cell Type Marker >> Neuron marker >> Soma marker
Applications
Show applications key
Our Abpromise guarantee covers the use of ab70218 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
IHC-P
IHC-Fr
- 1 Image
WB
Application notes
IHC-P: 1/300 - 1/3000. Antigen retrieval is recommended.
IHC-Fr: 1/300 - 1/3000.
WB: 1/300 - 1/3000. Detects a band of approximately >75 kDa (predicted molecular weight: 200 kDa).
Not yet tested in other applications.
Optimal dilutions/concentrations should be determined by the end user.
Target
Function
The exact function of MAP2 is unknown but MAPs may stabilize the microtubules against depolymerization. They also seem to have a stiffening effect on microtubules.
Sequence similarities
Contains 3 Tau/MAP repeats.
Post-translational
modifications
Phosphorylated at serine residues in K-X-G-S motifs by MAP/microtubule affinity-regulating kinase (MARK1 or MARK2), causing detachment from microtubules, and their disassembly (By similarity). MAP2A/c is phosphorylated. Phosphorylated upon DNA damage, probably by ATM or ATR. Isoform MAP2c is phosphorylated by FYN at Tyr-67.
Cellular localization
Cytoplasm > cytoskeleton.
Target information above from: UniProt accessionP11137
The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Alternative names
- DKFZp686I2148 antibody
- Dendrite specific MAP antibody
- DKFZp686I2148 antibody
see all
Database links
- Entrez Gene: 25595 Rat
- SwissProt: P20357 Mouse
- SwissProt: P15146 Rat
- Unigene: 256966 Mouse
- Unigene: 10484 Rat
Anti-MAP2 antibody images:
- MAP2 antibody (ab70218)
Immunohistochemistry of rat brain (corpus callosum) using ab70218 at 1/1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
- MAP2 antibody (ab70218)
Immunohistochemistry of rat brain (optical tract) using ab70218 at 1/1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
- MAP2 antibody (ab70218)
Immunohistochemistry of rat brain (cortex) using ab70218 at 1/1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
- MAP2 antibody (ab70218)
Immunohistochemistry of rat DRG using ab70218 at 1/500 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
- MAP2 antibody (ab70218)
Immunohistochemistry of rat spinal cord using ab70218 at 1/500 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
- MAP2 antibody (ab70218)
Immunohistochemistry of rat spinal cord (dorsal horn) using ab70218 at 1/1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
- MAP2 antibody (ab70218)
Immunohistochemistry of rat spinal cord (ventral horn) using ab70218 at 1/1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
- MAP2 antibody (ab70218)
Immunohistochemistry of rat spinal cord using ab70218 at 1/1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
Western blot - MAP2 antibody (ab70218)
All lanes : Anti-MAP2 antibody (ab70218) at 1/1000 dilution
Lane 1 : Rat brain lysate
Lane 2 : Rat brain lysate with immunising peptide
Predicted band size : 200 kDa
Observed band size : >75 kDa (why is the actual band size different from the predicted?)
- MAP2 antibody (ab70218)
Immunohistochemistry of rat trigeminal nerve using ab70218 at 1/1000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
- MAP2 antibody (ab70218)
Immunohistochemistry of rat spinal cord using ab70218 at 1/2000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
- MAP2 antibody (ab70218)
Immunohistochemistry of rat brain using ab70218 at 1/3000 dilution. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown).
References for Anti-MAP2 antibody (ab70218)
This product has been referenced in:
- Baltan S et al. Expression of Histone Deacetylases in Cellular Compartments of the Mouse Brain and the Effects of Ischemia. Transl Stroke Res 2:411-423 (2011). IHC-FoFr; Mouse.Read more (PubMed: 21966324) »
See 1 publication for this product
Publishing research using ab70218? Please let us know so that we can cite the reference in this datasheet
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"