Application
Western blot
Loading amount
20 µg
Gel Running Conditions
Reduced Denaturing
Sample
Human Cell lysate - whole cell (nerublastoma)
Specification
nerublastoma
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4% · Temperature: 16°C
Other product details
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 16°C · Diluent: 4% milk
Dilution
1/2000
Secondary antibody
Name
Non-Abcam antibody was used: anti-rabbit HRP linked antibody
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/4000
Detection
Detection method
ECL
Bands
Specific: 16 kDa
Exposure
40 second(s)
Additional data
Additional Notes
WB
membrane : PVDF
lysis buffer : Laemmil sample buffer (biorad)
Lane 1 : neuroblastoma
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
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投稿 Apr 28 2014