製品の概要

  • 製品名
  • 製品の詳細
    Rabbit polyclonal to MAGED1
  • 特異性
    This antibody is specific for MAGED1.
  • アプリケーション
    適用あり: IHC-P, WB, ELISAmore details
  • 種交差性
    交差種: Human
  • 免疫原

    A KLH conjugated synthetic peptide (10-30 aa in length) in the region of 466~481 of human MAGED1.

  • ポジティブ・コントロール
    • A549 lysate and breast carcinoma tissue.

製品の特性

  • 製品の状態
    Liquid
  • 保存方法
    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term.
  • バッファー
    Preservative: 0.09% Sodium Azide
    Constituents: PBS
  • Concentration information loading...
  • 精製度
    Protein G purified
  • 特記事項(精製)
    This antibody is purified through a protein G column and eluted out with both high and low pH buffers and neutralized immediately after elution then followed by dialysis against PBS.
  • ポリ/モノ
    ポリクローナル
  • アイソタイプ
    IgG
  • 研究分野

アプリケーション

Our Abpromise guarantee covers the use of ab38492 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

アプリケーション Abreviews 特記事項
IHC-P 1/50 - 1/100.
WB 1/100 - 1/500. Detects a band of approximately 86 kDa (predicted molecular weight: 86 kDa).
ELISA 1/1000.

ターゲット情報

  • 機能
    Involved in the apoptotic response after nerve growth factor (NGF) binding in neuronal cells. Inhibits cell cycle progression, and facilitates NGFR-mediated apoptosis. May act as a regulator of the function of DLX family members. May enhance ubiquitin ligase activity of RING-type zinc finger-containing E3 ubiquitin-protein ligases. Proposed to act through recruitment and/or stabilization of the Ubl-conjugating enzyme (E2) at the E3:substrate complex. Plays a role in the circadian rythm regulation. May act as RORA co-regulator, modulating the expression of core clock genes such as ARNTL/BMAL1 and NFIL3, induced, or NR1D1, repressed.
  • 組織特異性
    Expressed in bone marrow stromal cells from both multiple myeloma patients and healthy donors. Seems to be ubiquitously expressed.
  • 配列類似性
    Contains 1 MAGE domain.
  • 細胞内局在
    Cytoplasm. Cell membrane. Nucleus. Expression shifts from the cytoplasm to the plasma membrane upon stimulation with NGF.
  • Information by UniProt
  • 参照データベース
  • 別名
    • DLXIN 1 antibody
    • MAGD1_HUMAN antibody
    • MAGE D1 antigen antibody
    • MAGE tumor antigen CCF antibody
    • MAGE-D1 antigen antibody
    • Maged1 antibody
    • Melanoma antigen family D 1 antibody
    • Melanoma associated antigen D1 antibody
    • Melanoma-associated antigen D1 antibody
    • Neurotrophin receptor interacting MAGE homolog antibody
    • Neurotrophin receptor-interacting MAGE homolog antibody
    • NRAGE antibody
    • PP2250 antibody
    see all

画像

  • Anti-MAGED1 antibody (ab38492) at 1/100 dilution + A549 lysate

    Predicted band size : 86 kDa
    Observed band size : 86 kDa
    Additional bands at : 30 kDa,45 kDa,50 kDa,55 kDa,60 kDa,65 kDa. We are unsure as to the identity of these extra bands.
  • Formalin-fixed and paraffin-embedded human breast cancer tissue reacted with ab38492, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry.

参考文献

ab38492 has not yet been referenced specifically in any publications.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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