Anti-MADH7/SMAD7 抗体 (ab90086)
Key features and details
- Rabbit polyclonal to MADH7/SMAD7
- Suitable for: WB
- Reacts with: Human
- Isotype: IgG
製品の概要
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製品名
Anti-MADH7/SMAD7 antibody
MADH7/SMAD7 一次抗体 製品一覧 -
製品の詳細
Rabbit polyclonal to MADH7/SMAD7 -
由来種
Rabbit -
アプリケーション
適用あり: WBmore details -
種交差性
交差種: Human -
免疫原
Synthetic peptide corresponding to Human MADH7/SMAD7 (C terminal). NM_005904
Database link: O15105 -
ポジティブ・コントロール
- WB: HeLa cell lysate.
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特記事項
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
製品の特性
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製品の状態
Lyophilized:Reconstitute with 200ul distilled H2O. For extended storage, we suggest the addition of an equal volume of glycerol to make a final glycerol concentration of 50%. -
保存方法
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid repeated freeze / thaw cycles. -
バッファー
Preservative: 0.02% Sodium azide
Constituent: 1% BSA -
Concentration information loading...
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精製度
Immunogen affinity purified -
ポリ/モノ
ポリクローナル -
アイソタイプ
IgG -
研究分野
関連製品
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Compatible Secondaries
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Isotype control
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Recombinant Protein
アプリケーション
The Abpromise guarantee
Abpromise保証は、 次のテスト済みアプリケーションにおけるab90086の使用に適用されます
アプリケーションノートには、推奨の開始希釈率がありますが、適切な希釈率につきましてはご検討ください。
アプリケーション | Abreviews | 特記事項 |
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WB |
1/200 - 1/1000. Predicted molecular weight: 46 kDa.
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特記事項 |
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WB
1/200 - 1/1000. Predicted molecular weight: 46 kDa. |
ターゲット情報
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機能
Antagonist of signaling by TGF-beta (transforming growth factor) type 1 receptor superfamily members; has been shown to inhibit TGF-beta (Transforming growth factor) and activin signaling by associating with their receptors thus preventing SMAD2 access. Functions as an adapter to recruit SMURF2 to the TGF-beta receptor complex. Also acts by recruiting the PPP1R15A-PP1 complex to TGFBR1, which promotes its dephosphorylation. Positively regulates PDPK1 kinase activity by stimulating its dissociation from the 14-3-3 protein YWHAQ which acts as a negative regulator. -
組織特異性
Ubiquitous with higher expression in the lung and vascular endothelium. -
関連疾患
Colorectal cancer 3 -
配列類似性
Belongs to the dwarfin/SMAD family.
Contains 1 MH1 (MAD homology 1) domain.
Contains 1 MH2 (MAD homology 2) domain. -
翻訳後修飾
Phosphorylation on Ser-249 does not affect its stability, nuclear localization or inhibitory function in TGFB signaling; however it affects its ability to regulate transcription (By similarity). Phosphorylated by PDPK1.
Ubiquitinated by WWP1 (By similarity). Polyubiquitinated by RNF111, which is enhanced by AXIN1 and promotes proteasomal degradation. In response to TGF-beta, ubiquitinated by SMURF1; which promotes its degradation.
Acetylation prevents ubiquitination and degradation mediated by SMURF1. -
細胞内局在
Nucleus. Cytoplasm. Interaction with NEDD4L or RNF111 induces translocation from the nucleus to the cytoplasm (PubMed:16601693). TGF-beta stimulates its translocation from the nucleus to the cytoplasm. PDPK1 inhibits its translocation from the nucleus to the cytoplasm in response to TGF-beta (PubMed:17327236). - Information by UniProt
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参照データベース
- Entrez Gene: 4092 Human
- Omim: 602932 Human
- SwissProt: O15105 Human
- Unigene: 465087 Human
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別名
- CRCS3 antibody
- FLJ16482 antibody
- hSMAD 7 antibody
see all
画像
データシートおよび資料
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SDS download
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Datasheet download
参考文献 (26)
ab90086 は 26 報の論文で使用されています。
- Bai B et al. Molecular mechanism of the TGF‑β/Smad7 signaling pathway in ulcerative colitis. Mol Med Rep 25:N/A (2022). PubMed: 35137923
- Fu H et al. Downregulation of miR-17 suppresses TGF-ß1-mediated renal fibrosis through targeting Smad7. Mol Cell Biochem 476:3051-3064 (2021). PubMed: 33797702
- He D et al. miR-15a-5p regulates myocardial fibrosis in atrial fibrillation by targeting Smad7. PeerJ 9:e12686 (2021). PubMed: 35036160
- Guan R et al. Chitinase-like protein YKL-40 regulates human bronchial epithelial cells proliferation, apoptosis, and migration through TGF-ß1/Smads pathway. Hum Exp Toxicol 39:451-463 (2020). PubMed: 31797699
- Nowicki A et al. The Effect of 3'-Hydroxy-3,4,5,4'-Tetramethoxy -stilbene, the Metabolite of the Resveratrol Analogue DMU-212, on the Motility and Proliferation of Ovarian Cancer Cells. Int J Mol Sci 21:N/A (2020). PubMed: 32046103