Application
Immunocytochemistry/ Immunofluorescence
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 1% · Temperature: 25°C
Sample
Human Cell (HEK293, HeLa)
Specification
HEK293, HeLa
Permeabilization
Yes - 0.5% NP-40, 1%BSA in PBS
Fixative
Paraformaldehyde
Other product details
Incubation time
1 hour(s) and 0 minute(s) · Temperature: 25°C · Diluent: 0.5% NP-40, 1%BSA in PBS
Dilution
1/50
Secondary antibody
Secondary antibody
None used
Additional data
Additional Notes
Not good for in vivo
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.
DR. Monica Pons
Verified customer
投稿 Oct 01 2013