Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (HEK293)
Specification
HEK293
Fixative
100% EtOH
Permeabilization
Yes - 0.1% Triton X-100
Blocking step
Gelatin as blocking agent for 40 minute(s) · Concentration: 0.1% · Temperature: 25°C
Other product details
Dilution
1/1000
Incubation time
40 minute(s) · Temperature: 25°C · Diluent: 0/1% Gelatin in PBS
Secondary antibody
Name
Non-Abcam antibody was used: Invitrogen Probe - goat anti mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 594
Dilution
1/200
Additional data
Additional Notes
Fresh PBS containing 0.1% gelatin was used throughout as the wash solution. We are using this and other markers to look at the subcellular localization of APP dimerization. Cells were fixed with ice cold 100% EtOH for 30 min. All steps were carried out at room temperature.
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投稿 Jan 14 2011